Analysis of Growth Hormone Gene in Alpine and Saanen Goats Using PCR-SSCP Method
محورهای موضوعی : Camelر. خاتمی نژاد 1 , س. یوسفی 2 , م. آهنی آذری 3
1 - Department of Animal Science, Faculty of Agriculture, GonbadUniversity, Gonbad, Iran
2 - Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
3 - Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
کلید واژه: Goat, caprine GH gene, genetic polymorphism,
چکیده مقاله :
In this study the genetic polymorphism of growth hormone (GH) gene as a candidate gene in livestock was investigated. Blood samples were randomly collected from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 365 bp region of exon 5 of the GH genewas amplified by polymerase chain reaction (PCR). PCR products were analyzed using single strand conformation polymorphism (SSCP) method on polyacrylamide gel. The results indicated that there are four (G1, G2, G3 and G4) conformational patterns with frequencies 0.38, 0.21, 0.23 and 0.18 for Alpine goat and 0.48, 0.21, 0.17 and 0.14 for Saanen goat, respectively. These results revealed that GH gene was polymorph in this studyand showed that PCR-SSCP is an appropriate tool for detecting polymorphism and evaluating genetic variability.
در این مطالعه به منظور بررسی چند شکلی ژن هورمون رشد به عنوان یک ژن کاندیدا، نمونه‌های خون به طور تصادفی از 34 بز نژاد آلپاین و 42 بز نژاد سانن به دست آمد. DNA از نمونه‌های گرفته شده استخراج و ناحیه 365 جفت بازی از اگزون 5 ژن هورمون رشد توسط واکنش زنجیره‌ای پلی‌مراز تکثیر شد. محصولات PCR توسط روش SSCP بر روی ژل پلی اکریل آمید مورد آنالیز قرار گرفتند. نتایج آنالیز نشان داد که برای ژن مورد مطالعه چهار الگوی باندی متفاوت G1، G2،G3 و G4 به ترتیب با فراوانی‌های 38/0 ،21/0 ،23/0 و 18/0 در بز آلپاین و 48/0، 21/0 ، 17/0 و 14/0 در بز نژاد سانن مشاهده شدند.
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