Association of single nucleotide polymorphisms T886C in TAS2R38 gene and C109869T in SLC6A14 gene with Idiopathic infertility in Iranian men
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
elham siasi
1
(Islamic Azad University, North Branch Tehran)
ahmad aleyasin
2
(Medical genetic department, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran)
javad mowla
3
(Medical genetic department, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran)
Keywords: PCR-RFLP, sequencing, Idiopathic male infertility, Polymorphism, HRM-PCR Corbett,
Abstract :
Objective: Frequency of male infertility is 10-15% and there is not definite reason in 50% of infertility in men. This form of infertility is named idiopathic infertility and genetic factors are one of male infertility causes. Therefore, aim of this study was investigation on association between T886C polymorphism in TAS2R38 gene and C109869T polymorphism in SLC6A14 gene with Iranian idiopathic infertile male. Materials and Methods: For this study. DNA was extracted from 200 blood samples consist of 100 men with idiopathic infertility (oligospermic and azoospermia) and 100 fertile men as control groups. Genotyping of two studied polymorphisms were performed by using of molecular methods, HRM-PCR Corbett, PCR-RFLP and Sequencing, then results were statistical analyzed.Results: For T886C polymorphism in TAS2R38 gene statistical analysis showed no significant difference between patient and control groups (P=0.9) and results shown was not association between this polymorphism and idiopathic male infertility in Iranian population. Frequency of C109869T polymorphism in SLC6A14 gene was different in infertile patients and control groups (P=0.04) and is indicated significant relationship with idiopathic male infertility in studied Iranian men population.Conclusion: According to this research results, The C109869T polymorphism in SLC6A14 gene is actual in affected of idiopathic infertility in Iranian men population and could cause oligospermia and azoospermia and created infertility in Iranian male. For confirming these results is recommended study on more samples.
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