Effect of Hydroxyapatite Nanoparticles on Differentiotion of Mesenchymal Stem Cells into Bone cells in Polycaprolacton/Keratin/Hydroxyapatite Scaffolds
Subject Areas :Mahboobeh Mahmoodi 1 , Mina Kalantari 2 , Marjan Mirhaj 3
1 - Biomedical engineering department, Yazd Branch, Islamic Azad University
2 - Biomedical Engineering
3 - Biomedical Engineering
Keywords: "Electrospining", "Mesenchymal Stem Cells", "keratin", " Hydroxyapatite", "Nano fibers",
Abstract :
The human body process can spontaneously repair small bone defects, while large bone defects cannot be repaired without medical interventions. Efforts to eliminate these shortcomings have led to the foundation of bone tissue engineering. In this research, polycaprolactone/keratin scaffold and Polycaprolactone/keratin/hydroxyapatite scaffold were fabricated using electrospinning method and were evaluated. Then, in order to evaluate the cellular differentiation, adipose tissue-derived mesenchymal stem cells were cultured on scaffolds and the process of differentiation of mesenchymal stem cells into bone cells for 7 and 14 days was investigated by alkaline phosphatase and alizarin red test. Increase of the activity of the enzyme alkaline phosphatase, the presence of the minerals and the red-orange-color extended on the scaffolds containing hydroxyapatite nanoparticles that confirmed the presence of Ca + 2 , demonstrated the differentiation of mesenchymal cells into bone cells. Therefore, the results of this study suggest that the polycaprolactone/keratin/hydroxyapatite scaffold is a suitable substrate for proliferation and differentiation of stem cells for bone tissue engineering.
