Effects of Cytotoxicity of Nanoparticles of Ag/Si_O_P /Gelatin on Uterus Cancer Cell Lines (Hela)
Subject Areas : Journal of Animal Biologyسیده مبینا موسوی سروینه باغی 1 , عباسعلی دهپور جویباری 2 , صدف فرخنده 3
1 - گروه زیست شناسی، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران
2 - گروه زیست شناسی، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران
3 - گروه زیست شناسی، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران
Keywords: Nano particles, Ag/Si_O_P /gelatin, Uterus cancer, Hela cells class,
Abstract :
Nowadays, cancer is the second cause of mortality in the world and among its variants; the most common cancer in women is the ovary cancer. One of the methods for cancer treatment is chemical therapy. Some nanoparticles can be considered as a sensitizer because they enhance the cytotoxicity due to oxidative stress and increase the free radicals, especially reactive oxygen species (ROS), within cells resulting in cell death. In this study, elevated synergistic effect of Ag/Si_O_P /gelatin nanoparticles as radiosensitizer was evaluated in presence of cobalt-60 gamma rays on human ovary cancer cell line. In this experimental and laboratories study ,after duplication and farming the Hela cell class, gelatin silver nanoparticles with10, 20, 40, 60, 80, 160 densities are effected by Hela cancer cells, and after 72 hours of using MTT, the cytotoxicity is evaluated and the data are analyzed by Elisa Reader and Anova. The Results shown that silver nanoparticles of (p≤5%) 20, 40, 80 density after 72hours, the cell growth is significantly decreased according to controlled group. Silver nanoparticles of different sizes and density of this study is shown that gelatinous nanoparticle have effective cell relation or cancer cell (Hela) and highly induced apoptosis in cells and caused the death of cancer cell and have the lower poisonous to natural body cells.
