The Study of VEGF405CG Gene Polymorphisms Involved in Type II Diabetes Patients in the Population of Mazandaran Province
Subject Areas : Journal of Animal BiologyZ. Noroozi 1 , A.A. Dehpour Joybari 2
1 - Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
2 - Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
Keywords: polymorphism, gene, Type 2 diabetes, Population of Mazandaran Province, VEGF405CG,
Abstract :
Type 2 diabetes is the most common diabetes and includes about 90% of all diabetic cases in the world. The most common gene polymorphism (VEGF405CG) is in the untranslated region of 5UTR, 405CG polymorphism probably has an effect on the level of expression after the gene translation and increases the gene products. VEGF is a vascular endothelium factor that plays an important role in DR pathogenicity as an angiogenic and capillary permeability factor. Therefore, VEGF405CG may be a good candidate for potential of catching diabetes type 2. The purpose of this study was to determine the relationship between this gene and diseases such as type 2 diabetes in the population of Mazandaran province. The tested specimens consisted of 50 patient and 50 healthy people referring to the Atieh and Shahid Babaei laboratories. About 1 ml of blood containing EDTA (CBC) from 50 patient and 50 healthy individual was taken as a control after obtaining informed consent. In order to determine the amount and quality of DNA, which is a very important step in the RFLP method, spectrophotometric and electrophoresis method were used for quantitative and qualitative evaluation, respectively. To estimate the concentration of DNA, 4 μl of the base DNA solution was mixed with 1 μl of the sampler buffer and in a well, 1.5% agarose gel was evacuated in TAE buffer. A PCR product was evaluated on an2 % agarose gel. 5 μl of product of each reaction with 1 ml of color transferred to gel wells and electrophoresis was performed at a voltage of 100 V for 1.5 hours. The gel was stained in ethidium bromide solution (0.5 mg/ml) for 20 minutes and after transferred to distilled water was photographed with gel dock. This study does not show any association between VEGF405CG polymorphism among type II diabetic patients and further study is needed in different populations to better understanding the role of VEGF405CG.
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