Prevalence of sul genes in E.coli isolated from urinary tract infectious in Shahrekord
Subject Areas : microbiology
Mahnaz Shamaei
1
(M.Sc. Student of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)
Maryam Reisi
2
(Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)
Hossein Khodabandeh shahraki
3
()
Keywords: Antibiotic resistance, E. coli, Sulfonamides, Urinary tract infection ,
Abstract :
Urinary tract infections is one of the most common infectious diseases and E. coli is one of urinary tract infection the most important factor. The purpose of this investigation is prevalence of sul genes in E.coli isolated from urinary tract infectious in Shahrekord to form cross-sectional in 2013. Samples was prepared as sterile and in terms of urine tests, cultures and was studied. Investigation antimicrobial susceptibility was performed by disk diffusion method. As well as, for tracing Sul gene PCR reaction was performed in the presence of specific primers and the results was analyzed. In this study of 130 E. coli isolates studied 67 isolates (53/51%) resistance to co-trimoxazol was observed. The frequency of genes sul1, sul2 and sul3 was respectively 20/89%, 55/22% and 4/47%. In statistical analysis with chi-square test between to resistance sulfonamides and sul genes significant correlation was observed. The results showed that E. coli isolates are high resistant to sulfonamides that may be the indiscriminate use of these antibiotics.
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