Investigating the Effect of Collagen Electrospun Scaffold, Zinc Oxide Nano-particle Containing Stem Cells Derived from Adipose tTissue on Diabetic wWound in Rats

Subject Areas : Journal of Animal Biology

Ghazaleh Larijani ¹ , Kazem Parivar ² , Nasim Hayati Roodbari ³ , Parichehre Yaghmaei ⁴ , Naser Amini ⁵

1 - Department of Biology, Science and Research Unit, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Science and Research Unit, Islamic Azad University, Tehran, Iran
3 - Department of Biology, Science and Research Unit, Islamic Azad University, Tehran, Iran
4 - Department of Biology, Science and Research Unit, Islamic Azad University, Tehran, Iran
5 - Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

Received: 2024-05-20 Accepted : 2024-07-09 Published : 2025-01-12

Keywords: Collagen, Electrospun, Stem cells, Zink oxide Nano-particle, Diabetic wound,

Abstract :

Chronic wounds, including diabetic wounds, have poor healing, long-duration healing, and weak collagen deposition. Fabrication nanofiber scaffolds can have a higher surface-to-volume ratio for cell penetration and proliferation. Also, the addition of positively charged oxidized antibacterial agents can better penetration into the cells. In this study, we investigated the effect of collagen nanofibers along with zinc oxide Nano-particle and stem cells on diabetic wound. Collagen characterization was done using the FTIR test. Collagen/ZnO NP nanofibers were characterized by SEM, FTIR, swelling test, MTT, and cell adhesion techniques. The diameter of nanofibers was 05 ± 79 nm. Also, MTT and SEM images showed that cells on nanofibrous scaffolds combined with zinc oxide Nano-particle have better cell growth, proliferation, and adhesion. The results obtained from the animal and immunohistochemistry study also showed that the speed of wound healing and collagen production was better in the group with collagen, zinc oxide, and mesenchymal cells. Therefore, it can be said that the zinc oxide Nano-particle can be considered a new Nano-particle in electrospun fibers that increase cell viability and proliferation.

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