Antimicrobial activity of bacteriocin of lactic acid bacteria against betalactamase producing bacteria and evaluation of synergistic effect by some antibiotics

reyhaneh shirkavand ¹ ( Department of Microbiology, Biological Science College, Varamin-pishva branch, Islamic Azad University,Varamin-Pishva,Iran )
Fatemeh Noorbakhsh ² ( Department of Microbiology, Biological Science College, Varamin-pishva branch,Islamic Azad University,Varamin-Pishva,Iran )
sahar Honarmand Jahromi ³ ( Department of Microbiology, Biological Science College, Varamin-pishva branch, Islamic Azad University,Varamin-Pishva,Iran )

Submited date : 2024-06-18 Accepted date : 2024-07-29

Keywords: antimicrobial activity, bacteriocin, lactic acid bacteria, beta-lactamase, antibiotic,

Abstract :

Introduction: The purpose of this study was to investigate the antimicrobial activity of bacteriocins produced by lactic acid bacteria against beta-lactamase producing bacteria and to evaluate its synergism effect with some antibiotics. Method: In this study, bacteriocin was isolated from Lactobacillus acidophilus strains, and then the effect of produced bacteriocin against 3 pathogenic bacterial strains, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa was investigated by serial dilution method. The effectiveness of bacteriocin extract alone and in combination with commercial antibiotics against clinical pathogens was determined using the disk diffusion method in three replicates. Results: The results showed that all Pseudomonas aeruginosa and Acinetobacter baumannii strains were beta-lactamase positive. This study showed a statistically significant difference in bacteriocin-antibiotic synergism activity for ceftazidime, levofloxacin, and piperacillin in Pseudomonas aeruginosa strains and in Acinetobacter baumannii strains for tetracycline, gentamicin, and ceftazidime. Conclusion: Lactobacillus acidophilus bacteriocin along with some antibiotics showed synergism effect in beta-lactamase producing strains of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli.

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