Population frequency of Bifidobacterium longum and Bifidobacterium bifidum in the intestine of obese patients before and after gastric surgery

Subject Areas : microbiology

Kimia Golestanfar ¹ , Laya Takbiri Osgoei ² , Elham Siasi Torbati ³ , Hossein Khodabandeh shahraki ⁴

1 - Ph.D Student,Department of Microbiology, Faculty of Basic Siences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - Assistant Professor, Department of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, Iran
3 - Assistant Professor, Department of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, Iran.
4 -

Received: 2024-06-19 Accepted : 2024-08-02 Published : 2024-08-16

Keywords: Obesity, gastric surgery, Bifidobacterium longum, Bifidobacterium bifidum,

Abstract :

Introduction: Obesity is a chronic multifactorial disease related to genetics. Studies have shown that in obese people the quantity and quality of the intestinal microbiota population differs from that of healthy individuals. For further clarification in the present study, the populations of Bifidobacterium langum and Bifidobacterium bifidum were examined before and after bariatric surgery (either RYGB or Sleeve). Material and Methods: In this study, 30 obese individuals who were candidates for bariatric surgery using either RYGB or Sleeve particepated in the examination along with 18 healthy controls. After collection, fecal samples were extracted using a special kit. After ensuring the concentration and purity of the extracted DNA using real-time PCR method, the population of the above two bacteria in patient samples (before and after surgery) and healthy control samples were determined. Results: In the present study, the mean populations of Bifidobacterium longum and Bifidobacterium bifidum in the subjects before and after bariatric surgery in the control group were significantly higher than the other two groups (RYGB and Sleeve) (P <.05). In the RYGB group, the mean population of Bifidobacterium bifidum before bariatric surgery was higher than the Sleeve group. However, the mean population of this bacterium in the subjects after bariatric surgery in the Sleeve group was higher than the RYGB group (P <.05). Also, the population of Bifidobacterium longum before and after surgery in Sleeve group was higher than RYGB group (P <.05). In general, after bariatric surgery with both RYGB and Sleeve methods, the mean population of Bifidobacterium bifidum and Bifidobacterium langom decreased compared to before surgery and this decrease was more noticeable in RYGB group (P <.05). Conclusion: According to the results, it seems that weight loss through surgery can be effective in changing the quantity of bacteria in the intestinal microbiota.

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