In vitro evaluation of antifungal effect and morphological change due to essential oil of Cuminum cyminum on Fusarium verticillioides on isolated from Iran
Subject Areas : Journal of Comparative Pathobiology
علیرضا مختاری
1
(ندارد)
علیرضا خسروی
2
(ندارد)
تقی زهرایی صالحی
3
(ندارد)
Keywords: Fusarium verticillioides, Fumonisin, Cuminum cyminum, Micro dillution,
Abstract :
Fungi like Fusarium species that have generally been referred to as farm fungi, in terms oftoxicity and toxin production are prominent in their group. The most important fumonisin toxinis fumonisin B1. Fusarium toxins are generally produced at lower temperature than optimumgrowth temperature. Fumonisin is a toxin produced by Fusarium verticillioides. In recent yearsdue to ecological changes and increased soil nitrogen levels, fusarium contaminations haveincreased. In present study effect of essential oil (EO) of Cuminum cyminum on Fusariumverticillioides isolated in Iran were determined in vitro by micro dillution method.The main EO active ingredient of Cuminum cyminum is aldehydecuminic. This EO is watersoluble. This research was performed inon14 isolated. The drug sensitivity tests were performedaccording to standard methods Broth Micro dillution NLCCLS-M38A2. The tests were repeatedtwice.MIC (Minimum inhibition concentration) frequency of EO was 0.195 μg/mlwith42.84%susceptible isolate and the least frequent was in concentration0.293μg/mlwith7.14% susceptibleisolate. MFC (Minimum fungicidal concentration) frequency distribution was0.39 μg/ml with42.84% susceptible strains and lowest frequency of susceptible isolate were 0.146,0.293and0.586 μg/ml with 7.14%. The results indicate effectiveness of this essential oilinhibited the growth of Fusarium verticillioides. EO effect on transformation the fungus conidiawere proved by microscopic examination. MIC and MFC of cuminum cyminum EO on differentisolate of F. verticillioides were calculated 0.097-0.39μg/ml and 0.146-0.781μg/mlrespectively.
