Effect of clove oil essence loaded on iron nanoparticles on aminotransferase enzymes and some tissues in rainbow trout (Oncorhynchus mykiss)
Subject Areas : Journal of Comparative Pathobiology
مصطفی غفاری
1
(ندارد)
علی خسروانی زاده
2
(ندارد)
Keywords: Aminotransferase Enzymes, Anesthesia, Clove Oil Essence, iron nanoparticles, Rainbow trout,
Abstract :
In the present study 64 speciment of rainbow trout (Oncorhynchus mykiss) with a mean weight155.8±24 g and mean length of 23.7±2 cm randomly allocated in four groups. Fish of threegroups were immersed in water contained 10, 25, 50, and 100 ppm clove (Eugeniacaryophillata) oil essence loaded on iron nanoparticles to induce anesthesia in them, and fourthgroup was as indicator. Loss of equilibrium, light anesthesia, regaining equilibrium andcomplete recovery times were precisely recorded. Fish were transferred into separate tanks afteranesthesia, and the blood samples were collected by cutting of the caudal peduncle from 4groups at 3 and 24 hours after recovery times. Blood samples were centrifuged and thebiochemical indices determined in blood plasma included AST and ALT by spectrophotometer.Brain, kidney, branch, heart, lien and liver tissues were extracted and fixed in phosphate bufferfor histopathology. Results indicated that anesthetizing with different concentrations of clove oilessence loaded on iron nanoparticles have no significant effects on amounts ALT and AST inrainbow trout. Also microscopic study of prepared sections from different tissues showed noremarkable side effects. Because recommended dose for induction anesthesia in rainbow troutwas 10 ppm in this research, therefore fivefold of recommended have no side effect even. Acomparison result of this research by results of other researches is indicating that clove oilessence after loading on iron nanoparticles become more safe and effective.
