Determination of Diagnostic Value of Novavet ketone meter in the diagnosis of cows with subclinical ketosis

Subject Areas : Journal of Comparative Pathobiology

M.R Taheri Jazi ¹ , maryam karimi dehkordi ²

1 - D.V.M. Graduate, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - Department of clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Received: 2021-11-03 Accepted : 2021-11-03 Published : 2020-12-21

Keywords: Novavet ketone meter, Subclinical ketosis, Dairy cow,

Abstract :

One of the most important metabolic abnormalities in the early lactation period is ketosis. The gold standard test for the diagnosis of ketosis is the measurement of beta-hydroxybutyrate (BHB) in the blood. In this study, the diagnostic value of the electronic ketone meter, Novavet, for diagnosing cows with ketosis was evaluated in comparison with the standard laboratory method. Blood samples were taken from 68 cows in the period of 7-14 days after calving. In the laboratory, serum BHB was measured by spectrophotometry and Randox kit.  At the time of blood sampling, the amount of BHB in whole blood was determined with ketone meter in the same cows. BHB values obtained from the two methods were analyzed by T-test and Pearson correlation statistical models. In order to determine the best cut points of BHB using Novavet machine, which can make the best distinction between healthy and ketosis cows (based on reference test), also to estimate the sensitivity and specificity of Novavet, the ROC analysis is used. The amount of BHB in whole blood in the manual method is significantly higher than the laboratory method. However, there is a significant and good correlation between these two methods. Also, Novavet at the cut point of 1.2 mmol/l has the highest combination of sensitivity and specificity (100 and 41.9%) compared to the standard test. Due to the good and significant correlation between the Novavet test and its high sensitivity, it can be said that this method has an acceptable accuracy for diagnosing hyperketonemic cows.

References:

3. 
   
   
   
   1. 1. Duffield T. Subclinical ketosis in lactating dairy cattle. Veterinary clinics of North America: Food animal practice. 2000 Jul 1; 16(2):231-53.
   
   
   
   

    

   
   
   
   
   2. Gordon JL, LeBlanc SJ, Duffield TF. Ketosis treatment in lactating dairy cattle. The veterinary clinics of North America. Food animal practice. 2013 May 4; 29(2):433-45.
   
   
   
   

    

   
   
   
   
   3. Enjalbert F, Nicot MC, Bayourthe C, Moncoulon R. Ketone bodies in milk and blood of dairy cows: Relationship between concentrations and utilization for detection of subclinical ketosis. Journal of dairy science. 2001 Mar 1; 84(3):583-9.
   
   
   
   

    

   
   
   
   
   4. Youssef MA, El-Khodery SA, El-deeb WM, Abou El-Amaiem WE. Ketosis in buffalo (Bubalus bubalis): clinical findings and the associated oxidative stress level. Tropical animal health and production. 2010 Dec 1; 42(8):1771-7.
   
   
   
   

    

   
   
   
   
   5. Oetzel GR. Monitoring and testing dairy herds for metabolic disease. Veterinary Clinics: Food Animal Practice. 2004 Nov 1; 20(3):651-74.
   
   
   
   

    

   
   
   
   
   6. Weng X, Zhao W, Neethirajan S, Duffield T. Microfluidic biosensor for β-Hydroxybutyrate (βHBA) determination of subclinical ketosis diagnosis. Journal of nanobiotechnology. 2015 Dec 1; 13(1):13.
   
   
   
   

    

   
   
   
   
   7. LeBlanc S. Monitoring metabolic health of dairy cattle in the transition period. Journal of reproduction and Development. 2010; 56(S):S29-35.
   
   
   
   

    

   
   
   
   
   8. Voyvoda H, Erdogan H. Use of a hand-held meter for detecting subclinical ketosis in dairy cows. Research in veterinary science. 2010 Dec 1; 89(3):344-51.
   
   
   
   

    

   
   
   
   
   9. Iwersen M, Klein-Jöbstl D, Pichler M, Roland L, Fidlschuster B, Schwendenwein I, Drillich M. Comparison of 2 electronic cowside tests to detect subclinical ketosis in dairy cows and the influence of the temperature and type of blood sample on the test results. Journal of dairy science. 2013 Dec 1; 96(12):7719-30.
   
   
   
   

    

   
   
   
   
   10. Pichler M, Damberger A, Schwendenwein I, Gasteiner J, Drillich M, Iwersen M. Thresholds of whole-blood β-hydroxybutyrate and glucose concentrations measured with an electronic hand-held device to identify ovine hyperketonemia. Journal of dairy science. 2014b Mar 1; 97(3):1388-99.
   
   
   
   

    

   
   
   
   
   11. Doré V, Dubuc J, Bélanger AM, Buczinski S. Evaluation of the accuracy of an electronic on-farm test to quantify blood β-hydroxybutyrate concentration in dairy goats. Journal of dairy science. 2013 Jul 1; 96(7):4505-7.
   
   
   
   

    

   
   
   
   
   12. Pichler M, Damberger A, Arnholdt T, Schwendenwein I, Gasteiner J, Drillich M, Iwersen M. Evaluation of 2 electronic handheld devices for diagnosis of ketonemia and glycemia in dairy goats. Journal of Dairy Science. 2014a Dec 1; 97(12):7538-46.
   
   
   
   

    

   
   
   
   
   13. Hoenig M, Dorfman M, Koenig A. Use of a hand-held meter for the measurement of blood beta-hydroxybutyrate in dogs and cats. Journal of veterinary emergency and critical care. 2008; 18: 86-7.
   
   
   
   

    

   
   
   
   
   14. Byrne HA, Tieszen KL, Hollis S, Dornan TL, New JP. Evaluation of an electrochemical sensor for measuring blood ketones. Diabetes care. 2000 Apr 1; 23(4):500-3.
   
   
   
   

    

   
   
   
   
   15. Šimundić AM. Measures of diagnostic accuracy: basic definitions. Medical and biological sciences. 2008 Jan 1; 22(4):61.
   
   
   16. Whitaker DA, Smith EJ, Kelly JM. Some effects of nutrition and management on the fertility of dairy cattle. The Veterinary Record. 1993; 133(3): 61-64.
   
   
   
   

    

   
   
   
   
   17. Padilla L, Shibano KI, Inoue J, Matsui T, Yano H. Plasma vitamin C concentration is not related to the incidence of ketosis in dairy cows during the early lactation period. Journal of veterinary medical science. 2005; 67(9): 883-886.
   
   
   
   

    

   
   
   
   
   18. Greiner M, Pfeiffer D, Smith RD. Principles and practical application of the receiver-operating characteristic analysis for diagnostic tests. Preventive veterinary medicine. 2000; 45(1-2): 23-41.
   
   
   
   

    

   
   
   
   
   19. Mahrt A, Burfeind O, Voigtsberger R, Müller A, Heuwieser W. Evaluation of a new electronic handheld meter for measurement of β-hydroxybutyric acid in dairy cows. Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere. 2014; 42(1):5-10.
   
   
   
   

    

   
   
   
   
   20. Bach KD, Heuwieser W, McArt JA. Comparison of 4 electronic handheld meters for diagnosing hyperketonemia in dairy cows. Journal of dairy science. 2016 Nov 1; 99(11):9136-42.
   
   
   
   

    

   
   
   
   
   21. Carrier J, Stewart S, Godden S, Fetrow J, Rapnicki P. Evaluation and use of three cowside tests for detection of subclinical ketosis in early postpartum cows. Journal of dairy science. 2004 Nov 1; 87(11):3725-35.
   
   
   
   

    

   
   
   
   
   22. Pineda A, Cardoso FC. Validation of a handheld meter for measuring β-hydroxybutyrate concentrations in plasma and serum from dairy cows. Journal of dairy science. 2015 Dec 1; 98(12):8818-24.
   
   
   
   

    

   
   
   
   
   23. Fiorentin E, Tiecher A, Menegat C, Soares C, Aires A, Rocha R, Gonzalez FH. Accuracy of two hand-held electronic devices for determination of blood β-hydroxybutyrate in dairy cows. Revista Brasileira de Saúde e Produção Animal. 2017 Jul; 18(3):439-45.