Evaluation of probiotic effect of Lactobacillus Casei and Bacillus Coagulans on AGS and DU145 cancer cell line

Subject Areas : Journal of Comparative Pathobiology

سمانه Ansarinia ¹ , علی Sharifzadeh ²

1 - Department of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - Department of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Received: 2022-09-09 Accepted : 2022-09-09 Published : 2022-02-20

Keywords: Probiotic, Gastric cancer, Prostat Cancer,

Abstract :

Probiotics are a live microbial oral supplement and a range of different beneficial effects have been attributed to probiotics . Today, probiotics are recognized as a factor in preventing many infectious diseases and cancers. In this study, the effect of probioty lactobacillus casei and Bacillus coagulase on cancer cells (AGS, DU145) (gastric and prostate cancer cell line) was investigated. To do this, these two bacteria were cultured in Broth MRS medium and from 48, 24 and 72 hour cultures, sediment and supernatant were prepared. Supernatants were prepared in acidic and neutralized forms with 1N NaOH.. Concentrations of 100, 10 and 1000 μg / ml of extracts were also prepared separately on AGS and DU145 cancer cells cultured in 96-well microplate. The results showed that the concentrations of 1000 μg / ml of extract and 300 μl / ml of supernatant in 72 hours had inhibitory concentrations of more than 50%. The inhibitory effect of supernatants was directly related to time and concentration.

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