ارزیابی اثرات پروبیوتیک های Lactobacillus Casei و Bacillus Coagulans بر رده سلول های سرطانی AGSوDU145

محورهای موضوعی : پاتوبیولوژی مقایسه ای

سمانه انصاری نیا ¹ , علی شریف زاده ²

1 - گروه زیست شناسی، دانشکده علوم،واحد شهرکرد، دانشگاه آزاد اسلامی،شهرکرد، ایران
2 - گروه آموزشی میکروبیولوژی،دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی،شهرکرد، ایران

تاریخ دریافت : 1401/06/18 تاریخ پذیرش : 1401/06/18 تاریخ انتشار : 1400/12/01

کلید واژه: پروبیوتیک, سرطان معده, سرطان پروستات,

چکیده مقاله :

پروبیوتیکها مکمل خوراکی میکروبی زنده هستند و طیفی از اثرات مفید مختلف به پروبیوتیکها نسبت داده می شود. امروزه پروبیوتیک ها بعنوان عاملی برای پیشگیری از ابتلا به بسیاری از بیماری های عفونی و سرطان شناخته شده اند. در این تحقیق اثر پروبیوتیکیلاکتوباسیلوس کازئی و باسیلوس کوآگولانسروی سلولهای سرطانیAGS,DU145) ) رده سلول سرطانی معده وپروستات) بررسی شد . برای این کار ، این دو باکتری در محیط کشت Broth MRS کشت داده شده و از کشتهای 48،24 و 72 ساعته ، رسوب و سوپرناتانت تهیه گردید . سوپرناتانت ها در دوقالب اسیدی و خنثی شده با NaOH 1Nآماده سازی گردید . غلظتهای 100،10 و1000میکروگرم/میلی لیتر از عصاره ها نیزتهیه و بشکل مجزا روی سلولهای سرطانیAGSوDU145 کشت شده در میکروپلیت 96 خانه ای اثر داده شد. نتایج به دست آمده نشان داد که غلظت های1000 میکروگرم / میلی لیتر عصاره و 300 میکرولیتر/میلی لیتر سوپرناتانت در 72 ساعت دارای غلظت مهاری بیش از 50% بوده اند. اثر مهاری سوپرناتانت ها رابطه مستقیم بازمان و غلظت داشت.

چکیده انگلیسی:

Probiotics are a live microbial oral supplement and a range of different beneficial effects have been attributed to probiotics . Today, probiotics are recognized as a factor in preventing many infectious diseases and cancers. In this study, the effect of probioty lactobacillus casei and Bacillus coagulase on cancer cells (AGS, DU145) (gastric and prostate cancer cell line) was investigated. To do this, these two bacteria were cultured in Broth MRS medium and from 48, 24 and 72 hour cultures, sediment and supernatant were prepared. Supernatants were prepared in acidic and neutralized forms with 1N NaOH.. Concentrations of 100, 10 and 1000 μg / ml of extracts were also prepared separately on AGS and DU145 cancer cells cultured in 96-well microplate. The results showed that the concentrations of 1000 μg / ml of extract and 300 μl / ml of supernatant in 72 hours had inhibitory concentrations of more than 50%. The inhibitory effect of supernatants was directly related to time and concentration.

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