Investigation of the effects of copper sulfate on sperm quality parameters, DNA fragmentation rate and testicular tissue of adult rats of wistar breed

Subject Areas :

rahil jannatifar ¹ (Department of Reproductive Biology,Academic Center for Education,Culture and Research(ACECR),Qom,Iran)
hamid piroozmanesh ² (Department of Reproductive Biology,Academic Center for Education,Culture and Research(ACECR),Qom,Iran)
Leila naserpoor ³ (Department of Reproductive Biology,Academic Center for Education,Culture and Research(ACECR),Qom,Iran.)

Received: 2020-07-03 Accepted : 2020-09-20 Published : 2020-09-22

Keywords: Sperm quality, DNA Fragmentation, Testicular tissue, copper sulfate,

Abstract :

Aim: Copper sulfate is one of the most important environmental pollutants that has the ability to produce free radicals and create oxidative stress. The aim of this study was to investigate the effects of copper sulfate on sperm quality parameters, DNA fragmentation rate and testicular tissue of adult Wistar rats. Materials and Methods: In this experimental study, 30 adult Wistar rats weighing 250-200 grams were used. Random adult mice were treated in 3 control groups, copper sulfate receptor with concentration (100 mg / kg) and concentration (200 mg / kg) for 56 days. At the end of the treatment period, testicular weight, sperm count and parameters were assessed based on (WHO2010). The quality of sperm chromatin was assessed by acridine uranium nuclear pigments. Malondialdehyde level was measured. Data were analyzed using the One-Way ANOVA statistical method. Results: The quality of sperm parameters in copper sulfate with a concentration of 200 mg / kg decreased significantly (P <0.05). The testicular weight was significantly reduced at a dose of 200 mg / kg (P <0.05). The diameter of the seminiferous tubules, testosterone levels,, germ cell count, and sperm DNA fragmentation rate decreased at a dose of 200 mg / kg (P <0.05). The concentration of malondialdehyde at a dose of 200 mg / kg sulfate copper was significantly increased (P <0.05). Conclusion: This study shows that high concentrations of copper sulfate cause destructive effects on sperm quality, testicular tissue.

References:

1.Al-Fartusie, FS., Mohssan, SN. (2017). Essential trace elements and their vital roles in human body. Indian J Adv Chem Sci, 5(3);127-36.

2.Aliasgharpour, M. (2020). Trace elements in human nutrition (II)–an update. international Journal of Preventive Medicine, 1(1); 2-11. 3.Alonso, C., Casero, E., Román, E., Campos, SF-P., de Mele MFL. (2016). Effective inhibition of the early copper ion burst release by purine adsorption in simulated uterine fluids. Electrochimica Acta, 189; 54-63.

4.Ammar, O., Houas, Z., Mehdi, M. (2019). The association between iron, calcium, and oxidative stress in seminal plasma and sperm quality. Environmental Science and Pollution Research, 26(14); 14097-105.

5.Asif M. (2017). Role of heavy metals in human health and particularly in respect to diabetic patients. TANG [HUMANITAS MEDICINE], 7(1); 1.-.10.

6.Bataineh, H., Al-Hamood, M., Elbetieha, A. (1998). Assessment of aggression, sexual behavior and fertility in adult male rat following long-term ingestion of four industrial metals salts. Human & Experimental Toxicology, 17(10); 570-6.

7.D’souza, D., Subhas, BG., Shetty, SR., Balan, P. (2012). Estimation of serum malondialdehyde in potentially malignant disorders and post-antioxidant treated patients: A biochemical study. Contemporary Clinical Dentistry, 3(4); 448.

8.El-Hak, HNG., Mobarak, YM. (2020). Copper oxychloride–induced testicular damage of adult albino rats and the possible role of curcumin in healing the damage. Environmental Science and Pollution Research, 1-14.

9.Eliasson, R. (2010). Semen analysis with regard to sperm number, sperm morphology and functional aspects. Asian Journal of Andrology. 12(1);26.

10.Fraga, CG. (2005). Relevance, essentiality and toxicity of trace elements in human health. Molecular Aspects of Medicine, 26(4-5); 235-44.

11.Ghaniei, A., Eslami, M., BabaeiMarzango, SS. (2018). Determination of calcium, magnesium, phosphorus, iron, and copper contents in rooster seminal plasma and their effects on semen quality. Comparative Clinical Pathology, 27(2); 427-31.

12.Hultberg, B., Andersson, A., Isaksson, A. (1997). The cell-damaging effects of low amounts of homocysteine and copper ions in human cell line cultures are caused by oxidative stress. Toxicology, 123(1-2); 33-40.

13.Kilic, M. (2007). Effect of fatiguing bicycle exercise on thyroid hormone and testosterone levels in sedentary males supplemented with oral zinc. Neuroendocrinology Letters, 28(5); 681-5.

14.Kňažická, Z., Lukáčová, J., Greń, A., Formicki, G., Massányi, P., Lukáč, N. (2020). Relationship between level of copper in bovine seminal plasma and spermatozoa motility. Journal of Microbiology, Biotechnology and Food Sciences, 9(6); 1351-62.

15.Knazicka, Z., Tvrda, E., Bardos, L., Lukac, N. (2012). Dose-and time-dependent effect of copper ions on the viability of bull spermatozoa in different media. Journal of Environmental Science and Health, Part A, 47(9); 1294-300.

16.Naozuka, J. (2018). Elemental enrichment of foods: essentiality and toxicity. Nutri Food Sci Int, 4; 1-5.

17.Organization, WH. (2010). World health statistics 2010: World Health Organization, 2-10.

18.Ribas-Maynou, J., Yeste, M. (2020). Oxidative stress in male infertility: causes, effects in assisted reproductive techniques, and protective support of antioxidants. Biology, 9(4); 77.

19.Saleh, BOM. (2008). Status of zinc and copper concentrations in seminal plasma of male infertility and their correlation with various sperm parameters. Iraqi Academic Scientific Journal, 7(1); 76-80.

20.Shi, L., Song, R., Yao, X., Ren, Y. (2017). Effects of selenium on the proliferation, apoptosis and testosterone production of sheep Leydig Cells in Vitro. Theriogenology, 93; 24-32.

21.Slivkova, J., Popelkova, M., Massanyi, P., Toporcerova, S., Stawarz, R., Formicki, G. (2009). Concentration of trace elements in human semen and relation to spermatozoa quality. Journal of Environmental Science and Health Part A, 44(4); 370-5.

22.Sokol, RJ., Deverbaux, M., Mierau, GW., Hambidge, KM., Shikes, RH. (1990). Oxidant injury to hepatic mitochondrial lipids in rats with dietary copper overload: modification by vitamin E deficiency. Gastroenterology, 99(4); 1061-71.

23.Taylor, CT. (2001). Antioxidants and reactive oxygen species in human fertility. Environmental Toxicology and Pharmacology, 10(4); 189-98.

24.Torabi, F., Shafaroudi, MM., Rezaei, N. (2017). Combined protective effect of zinc oxide nanoparticles and melatonin on cyclophosphamide-induced toxicity in testicular histology and sperm parameters in adult Wistar rats. International Journal of Reproductive BioMedicine, 15(7); 403.

25.Victor, S., Richmond, RH. (2005). Effect of copper on fertilization success in the reef coral Acropora surculosa. Marine Pollution Bulletin. 50(11); 1448-51.

26.Wagner, H., Cheng, JW., Ko, EY. (2018). Role of reactive oxygen species in male infertility: An updated review of literature. Arab Journal of Urology, 16(1); 35-43.

27.Wu, W., Zhang, Y., Zhang, F. (1996). Studies on semen quality in workers exposed to manganese and electric welding. Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine], 30(5);266-8.