Immobilization of Bacillus alkalitelloris in agar for alpha-amylase enzyme production
Subject Areas : biochemistryMarjan Alimohamadiyan 1 , Mehdi Ebrahimi 2 , Sahar Honarmand Jahromi 3
1 - Islamic Azad University
2 - Department of Biochemistry and Biophysics, Faculty of Biological sciences, Varamin-Pishva Branch, Islamic Azad university, Varamin, IRAN
3 - Department of Microbiology, College of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
Keywords: Agar, Immobilization, α-Amylase, Bacillus alkalitelluris,
Abstract :
Due to the high consumption of amylase, the use of amylase-producing strains and optimizing the production conditions of this enzyme is an efficient way to produce this enzyme. Due to the fact that the amylase gene of Bacillus alkalitelluris has not been identified so far, it is important to achieve stable enzyme production conditions by the bacteria that produce these enzymes. One of the most important methods is to immobilization of the bacteria in suitable support so that the ability of these bacteria to produce enzymes is maintained. In this study, Bacillus alkalitelluris was first cultured in LB broth culture medium containing starch. The bacteria were then immobolized on agar and the amount of enzyme produced was measured using the DNS method. Finally, the amount of enzyme production when re-use stabilized cells in successive cultures was evaluated. According to the results, the bacterium Bacillus alkalitelluris immobilized in agar support is still able to produce alpha-amylase enzyme. Maximum alpha-amylase production was obtained after 24 hours. In addition, the immobilized bacteria remain after 4 times of changing the culture medium and are able to produce amylase enzyme so that up to 72 hours no significant change in enzyme production is observed. Due to the fact that the immobilized of Bacillus alkalitelluris in agar leads to the maintenance of alpha-amylase production capacity, so this method can be used in mass production of amylase enzyme.
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