Investigation of antibacterial effects of ethanolic extract of Sumac (Rhus coriaria L.) against Escherichia coli in vitro

Subject Areas : Food Science and Technology

حمدالله Moshtaghi ¹ , مریم Abbasvali ² , الناز Mohammadi ³ , A.R Safian ⁴ , میلاد Adel ⁵

1 - Assistant Professor, Department of Food Hygiene and Quality Control, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
2 - Assistant Professor, Department of Food Hygiene and Quality Control, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
3 - Graduated of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
4 - Graduated of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
5 - PhD student, Department of Aquatic Animal Health and Diseases, Caspian Sea Ecology Research Center, Sari, Iran

Received: 2013-05-04 Accepted : 2013-11-23 Published : 2013-08-23

Keywords: &lt, i&gt, Rhus coriaria L&lt, i&gt, , Antibacterial Effects, &lt, i&gt, Escherichia coli&lt, i&gt, ,

Abstract :

   The antibacterial effect of etbhanolic extract of Sumac (Rhus coriaria L.) was investigated quantitatively and qualitatively on Escherichia coli. The results of well diffusion test showed that extracts of Sumac in concentration of 0.5%, 1%, 2.5% and 5% could inhibited E. coli. In this study it was shown that MIC of the alcoholic extract of Sumac against E. coli was 6.25 mg/ml and its MBC against this bacterium was 50 mg/ml. The results from evaluation of the antibacterial effects of the Sumac revealed that at 4 and 15 °C, the growth of E. coli in test tubes containing meat extracts has increased Throughout the 48 h of incubation period. Results showed that the growth of this bacteria in different concentration of Sumac extract as decreased in the both tested temperatures in comparison to time zero (p<0.05). Furthermore, there was a significant difference in the number of microorganism at various times between control and experimental groups in both tested temperatures (p<0.05). 

References:

• Burt, S.)2004(. Essential oils: their antibacterial properties and potential applications in food-a review. Journal of Food Microbiology, 94(2): 223-253.


• Delgado, B., Palop, A. and Fenandez, PS. (2004). Combined effect of thymol and cymene to control the growth of Bacillus cereus vegetative cells. European Food Research and Technology, 218(3): 188-193.


• Fazeli, M.R., Amin, G., Ahmadian Attari, M.M., Ashtiani, H. and Samadi, N. (2007). Antimicrobial activities of Iranian sumac and a avishan-e shirazi (Zataria multiflora) against some food-borne bacteria. Food Control, 18(1): 646-649.


• Kalemba, D. and Kunicka, A. (2003). Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry, 10(1): 813 - 829.


• Kosar, M.B., Bozan, F. and Temelli, K. (2007). Antioxidant activity and phenolic composition of sumac (Rhus coriaria L.) extract. Food Chemistry, 103 (1): 952-959.


• Nasar-Abbas, S.M. and Kadir Halkman, A. (2004). Antimicrobial effect of water extract of sumac (Rhus coriaria L.) on the growth of some food borne bacteria including pathogens. International Journal of Food Microbiology,97(1): 63-69.


• Nasar-Abbas, S.M., Halkman, A.K. and Al-Haq, M.L. (2004). Inhibition of some food-borne bacteria by alcohol extract of sumac (Rhus coraria L.). Journal of Food Safety,24(1): 257-267.


• Ozcan, M. and Erkmen, O. (2001). Antimicrobial activity of the essential oil of Turkish plant spices. European Food Research and Technology, 212(1): 658-660.


• Politeo, O. and Jukic, M. (2007). Chemical composition and antioxidant capacity of free volatileaglycones from basil (ocimum basilicum L.) compared with its essential oil. Food Chemistry, 101(1): 379-385.


• Shahidi Bonjer, G.H., Karimi Nik, A. and Heydari, M.R. (2003). Antipsudomona and antibacilli activity of some medicinal plants of Iran. Daru Journal of Pharmaceutical Sciences, 11(1): 157-163.


• Reyna, S. and Mazza, G. (2007). Biological activities of extracts from sumac (Rhus spp.) a review. Plant Foods for Human Nutrition, 62: 165-175.


• Wetheritlt, H. and Pala, M. (1994). Herbs and spices in digenous to Turkey. Development in Food Sciences, pp. 258-307.