Experimental comparison of the effectsof ascorbic acid and thiamine in prevention of lead induced tissue damages in selected tissues of common carp (Cyprinus carpio)

Subject Areas : Veterinary Clinical Pathology

Fatemeh Pourali ¹ , Zahra Moosavi ² , Davar Shahsavani ³ , Mohammad Azizzadeh ⁴

1 - D.V.M. Graduate, Faculty of Veterinary Medicine, Ferdowsi University of Mashad, Mashad, Iran.
2 - Assistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashad, Mashad, Iran.
3 - Professor, Department of Fish Medicine, Faculty of Veterinary Medicine. Ferdowsi University of Mashad, Mashad, Iran.
4 - Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashad, Mashad, Iran.

Received: 2017-08-12 Accepted : 2019-01-02 Published : 2019-04-21

Keywords: Ascorbic acid, Lead, Histopathology, Common carp, Thiamine,

Abstract :

Due to accumulation of lead in edible tissues of fish, the safety and health of human food are also affected. This study was conducted to compare the effects of ascorbic acid and thiamine in prevention of lead induced tissue damages in selected tissues of common carp (Cyprinus carpio). Fish were divided randomly into 4 groups of 30 fish each. Group 1 was considered as control group. Group 2 received lead acetate while groups 3 and 4 received thiamine and ascorbic acid respectively in addition to lead. After necropsy, tissue specimens were collected from the brain, kidney and liver and the processed slides were stained with hematoxylin and eosin. Brain lesions in group 4 consisting of hyperemia, edema and ischemic cell changes were significantly lower in comparison to groups 2 and 3. In liver, hyperemia, hyperplasia of melano-macrophage centers, hepatocellular degeneration and intranuclear acid-fast inclusion bodies were observed. The severity of hyperemia and degeneration in group 3 was significantly lower in comparison to group 4. In the statistical comparison, none of the renal pathological indices including hyperemia, hemorrhage, intranuclear acid-fast inclusion bodies, necrotic changes, swelling of epithelial cells and hyperplasia of melano-macrophage centersrevealed significant differences between groups 3 and 4  but compared to group 2, some lesions in these groups showed a significant difference. Based on the results, it can be concluded that ascorbic acid and thiamin might have some protective and therapeutic effects on lead poisoning in fish.

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