Effect of berberine on polycyctic ovary syndrome induced by letrozole in adult female Wistar rats

Subject Areas : Veterinary Clinical Pathology

Saba Safdarpour ¹ , Akram Eidi ² , Pejman Mortazavi ³

1 - MSc of Animal Physiology, Department of Biology, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Professor, Department of Biology, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Received: 2023-09-27 Accepted : 2024-01-06 Published : 2023-10-23

Keywords: Rat, Berberine, Letrozole, Polycystic ovary syndrome,

Abstract :

Berberine which is the major active component of Berberis vulgaris, has been widely used in traditional medicine and has an antioxidant activity against oxidative stress. Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in reproductive-aged women. PCOS is often associated with a metabolic syndrome. In the present study, the effect of berberine on letrozole-induced PCOS was investigated in adult female Wistar rats. The rats were randomly divided into 8 groups: normal control, berberine (25, 50 and 100 mg/kg intragastrically, daily) alone, PCOS control rats (letrozole, 1 mg/kg, intragastrically, daily), berberine (25, 50 and 100 mg/kg intragastrically, daily) together with (letrozole 1 mg/kg, intragastrically, daily), and treatment was performed accordingly. Administration of berberine was started 30 minutes before the first dose of letrozole and continued up to 28 days. The animals were euthanized 24 hours after the last dose of the treatment. Antioxidant activity was tested by measuring the activity of superoxide dismutase, catalase and glutathione peroxidase enzymes as well as the amount of malondialdehyde in ovarian tissue homogenates. The findings of the present study showed that berberine increases antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase and decreases malondialdehyde levels. The results of this study suggest that berberine treatment may be beneficial in letrozole-induced PCOS in rats.

References:

• Amini, L., Tehranian, N., Movahedian, M., Ramezani Tehrani, F. and Ziaee, S. (2015). Antioxidant and management of polycystic ovary syndrome in Iran. Iranian Journal of Reproductive Medicine, 13(1): 1-8.


• Behboodi, Z., Fereidooni, B., Saffari, M. and Montazeri, A. (2018). Polycystic ovary syndrome and its impact on Iranian women's quality of life: a population-based study BMC. Womens Health, 18(1): 164.


• Bahman, M., Hajimehdipoor, H., Afrakhteh, M., Bioos, S., Hashem-Dabaghian, F. and Tansaz, M. (2018). The importance of sleep hygiene in polycystic ovary syndrome from the view of Iranian Traditional Medicine and Modern Medicine. International Journal of Preventive Medicine, 12(9): 87.


• Belgorosky, A., Guercio, G., Pepe, C., Saraco, N. and Rivarola, M.A. (2009). Genetic and clinical spectrum of aromatase deficiency in infancy. Childhood and adolescence. Hormone Research in Paediatrics, 72(6): 321-330.


• Chandirasegaran, G., Elanchezhiyan, C. and Ghosh, K. (2018). Effects of Berberine chloride on the liver of streptozotocin-induced diabetes in albino Wistar rats. Biomedicine and Pharmacotherapy, 99(10): 227-236.


• Davasaztabrizi, A. and Mehrani, K. (2017). Correlation of serum levels of T3 and T4 during the dry and postpartum periods with ovarian rebound in primiparous and multiparous cows. Journal of Veterinary Clinical Pathology, 11(41): 63-70. [In Persian]


• Di pietro, M., Abramovich, D., Parborell, F. and Pascuali, N. (2018). Ovarian angiogenesis in polycystic ovary syndrome. Reproduction, 155(5): 199-209.


• Firouzi, S. and Malekahmadi, M. (2018). Barberine in the treatment of obesity and metabolic syndrome: possible mechanisms of action. Diabetes, Metabolic Syndrome and Obesity, 8(11): 699-705.


• Gadalla, M.A., Huang, S., Wang, R., Norman, R.J., Abdullah, S.A., El Saman, A.M., et al. (2018). Effect of clomiphene citrate on endometrial thickness, ovulation, pregnancy and live birth in anovulatory women: systematic review and meta-analysis. Ultrasound in Obstetrics & Gynecology, 51(1): 64-76.


• Gonzalez, F. (2012). Inflammation in polycystic ovary syndrome underpinning of insulin resistance and ovarian dysfunction. Medicina Intensiva, 77(4): 300-305.


• Ghanbari, F., Shiravi, A., Molzemi, S. and Kalalian Moghadda, H. (2012). Effect of berberine hydrochloride on testicular damage in streptozotocin-induced diabetic rats. International Journal of Environmental Research and Public Health, 7(3): 129-135.


• Hassani-Bafrani, H., Najaran, H., Razi, M. and Rashtbari, H. (2019). Berberine ameliorates experimental varicocele-induced damages at testis and sperm levels; evidences for oxidative stress and inflammation. Dalton Trans, 51(2): 497-485.


• Kaveh, A., Haghdoust, R. and Mosaferi, S. (2015). Comparison between epidural injection of calcium–naloxone and intramuscular GnRH as therapeutic methods in the treatment of follicular ovarian cysts in dairy cattle. Journal of Veterinary Clinical Pathology, 2(30): 435-444. [In Persian]


• Kalhori, Z., Soleimani Mehranjani, M., Azadbakht, M. and shariaatzadeh, M.A. (2018). Ovary stereological features and serum biochemical factors following induction of polycystic ovary syndrome with testosterone enanthate in mice: an experimental study. International Journal of Reproductive BioMedicine, 16(4): 267-274.


• Li, MF., Zhou, X.M. and Li, X.L. (2018). The effect of berberine on polycystic ovary syndrome patients with insulin resistance (PCOS-IR): A meta-analysis and systematic review. Evidence-Based Complementary and Alternative Medicine, 32(5): 253-261.


• Marshall, K. (2001). Polycystic ovary syndrome: clinical consideration. Alternative Medicine Review, 6(3): 272-292.


• Mohajeri, D., Mousavi, G., Kaffashielahi, R. and Neshatgharamalek, M. (2017). Study on protective effect of naringenin (Citrus flavonone) on incipient diabetic hepatopathy in alloxan-induced diabetic rats. Journal of Veterinary Clinical Pathology, 1(37): 39-52. [In Persian]


• Qadri, S., Hussain, A. and Bhat, M.H. (2018). Polycystic ovary syndrome in bipolar affective disorder: a hospital-based study. Indian Journal of Psychological Medicine, 40(2): 121-128.


• Saleh, S. R, Attia, R. and Ghareeb, D. (2018). The ameliorating effect of berberine-rich fraction against gossypol-induced testicular inflammation and oxidative stress. International Journal of Women's Health, 3(10): 1056-1061.


• Wang, H.S. and Wang, T.H. (2003). Polycystic ovary syndrome insulin resistance and insulin like growth factors, IGF binding proteins. Chang Gung Medical Journal, 26(8): 540-553.


• Tabeshpour, J., Imenshahidi, M. and Hosseinzadeh, H. (2017). A review of the effects of Berberis vulgaris and its major component, berberine, in metabolic syndrome. Iranian Journal of Basic Medical Sciences, 20(5): 557-568.


• Zan, Y., Kuai, C.X., Qiu, Z.X. and Huang, F. (2017). Berberine ameliorates diabetic neuropathy: TRPV1 modulation by PKC pathway. The American Journal of Chinese Medicine, 45(8): 1709-1723.


• Zhifang, X., Wei, F., Qian, S., Nannan, Y.U., Kun, Y.U., Shenjun, W., et al. (2017). Rhizoma Coptidis and berberine as a natural drug to combat aging and aging-related diseases via anti-oxidation and AMPK activation. Aging and Disease, 8(6): 760-777.