The effect of different levels of garlic extract administration at various time periods on the extent of serumic lipid peroxidation in laying hens

Subject Areas : Veterinary Clinical Pathology

saeed rasoulinejad ¹ (دانشگاه آزاد اسلامی، واحد گرمسار، دانشکده دامپزشکی، دانش آموخته دکترای حرفه ای دامپزشکی، گرمسار، ایران)
nariman sheykhi ² (دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، استادیار دانشکده دامپزشکی، گروه علوم درمانگاهی، تهران، ایران)
hossein hassanpour ³ (دانشگاه شهرکرد، استادیار دانشکده دامپزشکی، گروه علوم پایه، شهرکرد، ایران)

Received: 2012-10-25 Accepted : 2013-01-30 Published : 2013-02-19

Keywords: Lipid peroxidation, laying hens, garlic extract,

Abstract :

Free radicals are involved in molecular changes and mutation and if they are not inactivated the resultant lipid peroxidation will damage all types of cellular macromolecules such as proteins, carbohydrates, lipids and nucleic acids. Natural diet containing antioxidants plays an important role in health, reproduction, performance, safety and growth in poultry. Garlic extract with a high antioxidant effect very useful against free radicals and infective agents. In this study, the effect of concentration of 0.01 and 0.02% of garlic extract in drinking water with periods of 2, 4 and 6 days a week were evaluated on lipid peroxidation. In addition to assessing the best concentration, economic aspects were also considered. So at the end of the third week, the best effect on reducing lipid peroxidation was seen in T3 which had received the concentration of 0.02% of garlic extract, two days a week.  After six weeks of trial, Group T7 which had received the concentration of 0.02% garlic extract for 6 days a week, showed the best effect on reducing lipid peroxidation. Our studies also showed that garlic extract improved mass production in all groups compared to the control group.  Considering the amount of lipid peroxidation at the end of the sixth week, herd performance in six weeks and calculating the average sale price of mass production, minus the cost of feed consumed per group, it is concluded that group T4 which received garlic extract concentration of 0.01% for 4 days a week (Saturday, Monday, Wednesday, Thursday) showed the best option in view of the economic conditions.

References:

• Ayaz, E. and Alpsoy, H.C. (2007). Garlic (Allium sativum) and traditional medicine. Acta Parasitol Turcica, 31 (2):145-149.


• Baytop, T. (1999). The treatment Plant in turkey. Nobel Tip Kitabevie, Istanbul. ISBN: 9754200211.


• Borek, C. (2001). Antioxidant health effects of aged Garlic extract. Journal of Nutrition, 131(3s):1010-1015.


• Chowdhury, S.R., Chowdhury, S.D. and Smith, T.K. (2002). Effects of Dietary Garlic on Cholesterol Metabolism in Laying Hens. Poultry Science, 81:1856-1862.


• Djeridane, M., Yousfi, B. and Nadjemi, D. (2006). Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97(4):654-660.


• Ebadi, A., Rahimielenji, A., Taghadosi, M., Khorshidi, A. and Akbri, H. (2007). Effect of garlic on blood glucose in type 2 diabetic patients. Quarterly Journal of Feiz, 11(1):76-85 [In Farsi].


• عبادی، ع.، رحیمی لنجی، ا.، تقدسی، م.، خورشیدی، ا. و اکبری، ح. (1386). تاثیر قرص سیر بر قند خون بیماران دیابتی نوع 2. فصلنامه علمی  پژوهشی فیض، دوره یازدهم، شماره 1، صفحات 85-76.


• Filomeni, G., Aquilano, K., Rotilio, G. and Ciriolo, M.R. (2003). Reactive oxygen species-dependent c-Jun NH2-terminal kinase/c-Jun signaling cascademediates neuroblastoma cell death induced by diallyl disulfide. Cancer Research, 63(18):5940-5949.


• Ide, N., Lau, B.H. and Ryu, K. (1999). Antioxidant effects of fructosyl arginine, a Maillard reaction product in aged garlic extract. Journal of Nutrition Biochemistry, 10(6):372-376.


• Isabella, D., Ranieri, R., Colombo, D. and Aldo, M. (2006). Biomarkers of Oxidative Damage in Human Disease. American Association for Clinical Chemistry, 52(4):601-623.


• Sahin, K., Sahin, N. and Yaralioglu, S. (2002).Effects of Vitamin C and Vitamin E on Lipid Peroxidation, Blood Serum Metabolites, and Mineral Concentrations of Laying Hens Reared at High Ambient Temperature.Biological Trace Element Research, 85(2):321-328.


• Leonard, T., Rael Gregory, W., Thomas, M., Craun, C., Gerald, C., Bar-Or, R. and Bar-Or, D. (2004). Lipid Peroxidation and the Thiobarbituric Acid Assay: Standardization of the Assay When Using Saturated and Unsaturated Fatty Acids.Journal of Biochemistry and Molecular Biology, 37(6):749-752.


• Proctor, H.P. (1989). CRC Handbook on free radical and antioxidants. Vol.1. pp 209- 221.


• Rafieyan, M., Taji, F., Shirzad, H., Ashrafi, K., Parvin, N. and Kheiri, S. (2010). Comparison of the antioxidant activity of fresh and aged garlic extract. Zahedan Journal of Medical Sciences, 8(2): 112-118 [In Farsi].


• رفیعیان، م.، تاجی، ف.، شیرزاد، ه.، اشرفی، ک.، پروین، ن. و خیری، س. (1389). مقایسه قدرت آنتی اکسیدانی عصاره های سیر تازه و کهنه. مجله تحقیقات علوم پزشکی زاهدان، دوره 8، شماره 2، صفحات 118-112.


• Sahin, K., Onderci, M., Sahin, N., Gursu, M.F. and Kucuk, O. (2003). Dietaryvitamin C and folic acid supplementation ameliorates the detrimental effects of heat stress in Japanese quail. Journal of Nutrition, 133:1882-1888.


• Stanacev, V., Glamocic, D. Milosevic, N., Puvaca, N., Stanacev, V. and Plavsa, N. (2011). Effect of garlic (allium sativum l.)  in fattening chicks nutrition. African Journal of Agricultural Research, 6(4):943-948.


• Surai, F. (2007). Natural Antioxidant in poultry nutrition, new development. 16th European Symposium on Poultry Nutrition.


• Telugu, A., Raju, N., Lakshmi, A.N., Anand, T., Rao, L.V. and Sharma, G. (2000). Protective effects of quercetin during influenza virus-induced oxidative stress. Asia Pacific Journal Clinical Nutrition, 9(4):314-317.


• Tesoriere, L., D’Arpa, D., Butera, D., Pintaudi, A.M., Allegra, M. Livrea, M.A. (2002). Exposure to malondiadehyde induces an early redox unbalance preceding membrane toxicity in human erthrocytes. Free-Radi Research, 36:89-97.