Study on Changes of Atropine and Scopolamine Content and Growth Characteristics of Atropa belladonna L. affected by Bio-fertilizers and Chemical-fertilizers

Subject Areas : Medicinal Plants

Mohammad Inanloo Far ¹ (Dept. Agronomy, Shahrood University of Technology, Shahrood, Iran.)
Hassanali Naghdi Badi ² (Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran)
Mostafa Heidari ³ (Dept. Agronomy, Shahrood University of Technology, Shahrood, Iran.)
Majid Tolyat Abolhasani ⁴ (Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran)
Hassan Makarian ⁵ (Dept. Agronomy, Shahrood University of Technology, Shahrood, Iran)
Mohammad Reza Amerian ⁶ (Dept. Agronomy, Shahrood University of Technology, Shahrood, Iran)

Received: 2019-08-09 Accepted : 2019-12-16 Published : 2020-02-20

Keywords: plant growth-promoting rhizobacteria, atropine, Scopolamine, Alkaloids, Atropa belladonna L,

Abstract :

  Abstract Nowadays, the use of Plant Growth Promoting Rhizobacter (PGPR) as a biofertilizers is one of the ways to improve growth and phytochemical characteristics of medicinal plants. This study was carried out as a factorial experiment based on randomized complete block design with a randomized complete block design with 3 replications in the research farm of the Institute of Medicinal Plants - ACECR in 2017.The growth-promoting bacteria as a first factor were non-inoculation, Pseudomonas, Azotobacter, Pseudomonas + Azotobacter, and Thiobacillus + Sulfur. The chemical fertilizers as a second factor were no fertilizer or control, 50% recommended fertilizer and 100% recommended fertilizer. At flowering stage, the alkaloids were extracted by using chloroform, methanol and ammonia solvents. The amount of atropine and scopolamine was measured by High-performance liquid chromatography (HPLC). The biological and chemical fertilizers, as well as their interaction effect had significant effect (p<0.01) on growth traits, atropine and scopolamine content of leaf and root. The maximum biological yield was observed in Pseudomonas with 100% of recommended fertilizer. The highest amount of leaves atropine and scopolamine was related to Pseudomonas with 50% fertilizer and non-biofertilizer treatment with 50% recommended fertilizer, respectively. The highest amount of root atropine was related to Pseudomonas without chemical fertilizer application. The highest content of root scopolamine was observed in treatment of Pseudomonas with 100% recommended. Therefore, the highest biological yield and the amount of atropine in leaves and roots, as well as the scopolamine content of the root were obtained using Pseudomonas application.

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