Background & Aim:Anise studies have demonstrated different properties such as anti-cancer, anti-fungal, antibacterial, antioxidant, strengthen the liver, gastrointestinal tract, wounds heal and strengthen the nerve cells. Outbreaks of listeriosis in the general population are 7.0 per 100,000 populations. Pregnant women, infants, the elderly and immunocompromised patients or those weaknesses comprise most cases. The aim of the present study was to investigate the effect of intravenous and oral administration of anise herb to BALB/c race of mice with listeriosis. Experimental: 2 units of listeria monocytogenes were prepared in 0.5 Mc Farland concentrations and injected to BALB/c trace mouse intraperitoneal. Before and after treatment, the mice were examined for agglutination test.Results: The results of this study indicated that the effect of oral treatment with aqueous extract is better than injection of essential oil of anise. Recommended applications/industries: According to results of the present study, anise aqueous extract was recommended as a potential antibacterial agent against listeria monocytogenes. Manuscript profile