BACKGROUND: Chickpea is the second most important legume with numerous economic and nutraceutical properties. Therefore, chickpea production needs to be increased from the current level. OBJECTIVES: Considering that the amount of nutrients in the seeds of each plant is one of its positive nutritional characteristics, this research was conducted to investigate the role of iron and cytokinin on the increase of iron, zinc and manganese elements. METHODS: This research was conducted in a factorial experiment using randomized complete blocks design (RCBD) with four replications in Grit Agricultural Services Center located in Eastern-North of Khorramabad of lorestan province in 2011-12 and 2012-13 cropping seasons. The treatments consisted of three cultivars: (Hashem, Azad and local mass), four levels of iron application [control (lack of consumption), 5, 10 and 15 ppm] and four levels of cytokinin (6-Benzyl aminopurin) [control (lack of consumption), 5, 10 and 15 ppm]. RESULT: The reaction of cultivars regarding the accumulation of iron, zinc and manganese elements in the seed was different compared to iron and cytokinin. The effect of iron and cytokinin was positive on the accumulation of iron in the seed, and the highest amount of iron (with an average of 72.7 mg.kg-1) belonged to the Hashem cultivar and the application of 10 mg.l-1 of iron and 15 mg.l-1 of cytokinin. While with the increase of iron and cytokinin concentration, the amount of zinc and manganese accumulation in the seed decreased and the highest amount of zinc and manganese accumulation was obtained by usage of 5 ppm of iron and cytokinin. CONCLUSION: The highest seed yield belonged to the local mass with a concentration of 15 mg.l-1 of iron and cytokinin with an average of 179.6 grams per square meter. Manuscript profile