Introduction: Trimethyltin is a methylated organotin, which induces selective damage and neuronal death in human and rodents’ CNS. On the other hand, the neuroprotective effects of Gallic acid can prevent toxicity of trimethyltin. In the current study, the repairing effect of Gallic acid in cell injury caused by trimethyltin in rats was evaluated. Also, this study assessed the effects of Gallic acid on neuronal density of the different hippocampal areas of intoxicated rats with trimethyltin. Methods: 30 rats were divided in three groups: the no treatment control group, the control group, and the experimental group with intraperitoneal injection of trimethyltin (8 mg/kg) and Gallic acid solvent for 14 days. In the experimental group, after the administration of trimethyltin, 50 mg/kg of Gallic acid was injected. It was continued for 14 days. Finally, the rats were killed with transcardial perfusion. Histopathological and stereological analysis was performed on the rats. In this research, Kolmogorov-Smirnov test, one-way analysis of variance, and Tukey’s post-hoc test were used. Results: The results of this study showed that neuronal density of different hippocampal areas of the no treatment control group was significantly increased compared to the rats of the control and experimental groups (P<0.05), while chronic administration of Gallic acid could prevent apoptosis and protect hippocampal cells. Conclusions: According to the results, it is suggested that chronic administration of Gallic acid decreases the effects of trimethyltin and therefore, it prevents the reduction of hippocampal cells. Manuscript profile

Introduction: Gestational diabetes mellitus is a multi-factor disease. Several genes are shown to be associated with diabetes mellitus. Among them, IRS-1 gene is located on chromosome No.2 which is an endogen substrate for insulin receptor. IRS-1 gene has an important role in insulin pathway signaling. Aim of this study was to review the association of Ala513Pro and Gly972Arg IRS-1 gene polymorphism with gestational diabetes mellitus in south of Iran. Methods: 200 subjects with gestational diabetes mellitus and 200 healthy subjects as control group were enrolled in the study. DNA was extracted from their blood and polymorphism of G, C and T allele were studied. It was aimed to investigate whether genotype is associated with clinical and biochemical variables such as family history, fasting blood sugar, blood lipid and body mass index. Independent sample t- test, chi- square test, logistic regression and Hardy- Weinberg equilibrium tests were used to analyze data (p≤0.05). Results: The finding from the Polymorphism Gly972Arg study show that individuals with CC, CT genotypes were associated with an increased risk of the disease (%95CI: 0.02-0.44, OR=0.1, P<0.001), (%95CI:2-58.43, OR=9.2, P<0.01). There was no allele and genotypic variation in Polymorphism Ala513Pro Polymorphism. Conclusion: Based on the obtained results, Ala 513Pro polymorphism of the IRS-1 gene was not correlated, but Gly 972 Arg polymorphism related with gestational diabetes mellitus in population of south Iran. Manuscript profile

Introduction: Infertility is defined by the failure to reach a clinical pregnancy after one year of unprotected sexual intercourse, and often 15% of couples are infertile. Male factor solely and partially contributes to about 20% and 50% of infertilities, respectively. Both environmental and genetic factors play important roles in infertility, and the mybl1 gene and its polymorphisms are well known in this case. This study was going to evaluate whether rs7008740 and rs11987254 polymorphisms in mybl1 gene have a significant relationship with the risk of male infertility in Fars province or not.
Materials and methods: This study involved 100 infertile men and 100 fertile men (control group). The Tetra-ARMS PCR and ARMS PCR techniques were used to detect mybl1 polymorphism genotypes.
Results: The results showed that rs7008740 and rs11987254 polymorphisms in mybl1 gene did not have a significant relationship with the risk of male infertility in this population (P>0.05). However, there was a significant relationship between family history and infertility (P <0.001).
Conclusion: This study indicated that rs7008740 and rs11987254 polymorphic loci in mybl1 gene did not have a significant relationship with the risk of male infertility. Manuscript profile