Influence of Fibrolytic Enzymes on the in vitro Hydrolysis and Fermentation of Different Types of Roughages Treatment
محورهای موضوعی : Camel
A. Moharrery
1
(Department of Animal Science, FacultyofAgricultur, Shahrekord University, Shahrekord, Iran)
کلید واژه: alkaline treatment, fibrolytic enzymes, roughages, <i>in vitro</i> degradation,
چکیده مقاله :
The effects of pre-treating different types of roughages with alkali on the efficacy of exogenous fibrolytic enzymes for improving their digestibility were studied in vitroin factorial arrangement 4 × 3 × 5 (enzyme, treatment and roughage types). Two fibrolytic feed enzymes novozyme (N) and celloclast (C) their combination (N+C) were evaluated for their potential to improve in vitrodegradation of different roughages including two straws (wheat straw: WS; barley straw: BS), grass hay (GH), corn stover (CS) and corn cobs (CC) as untreated or alkaline treated (NaOH or urea). The enzyme products were in liquid form and applied at a rate of 1 mg enzyme protein/g DM substrate. Anaerobic buffer medium and strained ruminal fluid were added to the in vitroincubations. Degradabilities of dry matter (DM), neutral detergent fibre (NDF), cellulose, soluble protein and reducing sugar profiles were determined at the end of the 24 h incubation. Overall NDF degradability of roughages were slightly increased by alkaline treatment (P<0.05) but, the addition of fibrolytic enzymes directly to the ruminal fluid at the application rates could not increase significantly DM or cellulose degradation (P>0.05). Use of fibrolytic enzymes released higher amounts of reducing sugars and lower soluble protein compared to control (P<0.05). Alkali treatment increased (P<0.05) the degradability of DM and fiber degradability. It was higher in NaOH treated forage compared to urea treated forages (P<0.05).
تأثیرآنزیم‌های با منشاء خارجی و هیدرولیزکننده الیاف، بر روی چند نوع ماده خشبی که با استفاده از مواد قلیایی فرآوری شده بودند به منظور بررسی قابلیت هضم آنها در یک آزمایش فاکتوریل 5 × 3 × 4 (آنزیم، ماده قلیایی و ماده خشبی) مورد مطالعه قرار گرفت. دو آنزیم خوراکی با نام‌های نووزایم N و سلوکلست C و ترکیب مساوی این دو N + C به منظور ارزیابی توان تجزیه‌کنندگی آنها در شرایط آزمایشگاهی بر روی مواد حجیمی شامل 2 نوع کاه (گندم WS، جو BS)، علوفه غلات GH، پوشش برگی بلال CS و چوب بلال CC مورد بررسی واقع شد. این مواد حجیم قبلاً توسط سود و یا اوره فرآوری شده بودند. مایع شکمبه همراه با بافر و مواد حجیم تحت آزمون، مخلوط و برای مدت 24 ساعت در شرایط بی‌هوازی انکوبه شدند. تجزیه‌پذیری ماده خشک DM، دیواره سلولی NDF، سلولز و غلظت پروتئین محلول و قندهای احیاء موجود در محیط عمل بعد از گذشت 24 ساعت اندازه‌گیری شد. به طور کلی در همه موارد فرآوری مواد حجیم با سود سبب افزایش تجزیه‌پذیری NDF شد (05/0P<). اما افزودن آنزیم به طور مستقیم به مایع شکمبه نتوانست بهبودی در تجزیه‌پذیری DM و سلولز بوجود آورد (05/0P>). استفاده از آنزیم توانست غلظت قندهای احیاء و پروتئین محلول را نسبت به گروه کنترل افزایش دهد (05/0P<). فرآوری قلیایی نیز توانست تجزیه‌پذیری DM و الیاف را بهبود بخشد (05/0P<). البته این بهبود، در فرآوری با سود بیشتر از فرآوری با اوره مشاهده شد.
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