Optimizing Chia (Salvia hispanica L.) Cultivation in Iran Regions: A Study on Planting Density and Growth Regulator Effects

محورهای موضوعی : Plant Physiology

Fatemeh Javadifard ¹ , Khodayar Hemmati ² , Nastaran Hemmati ³

1 - Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 - Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 - Department of Horticultural Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.د

تاریخ دریافت : 1403/04/02 تاریخ پذیرش : 1403/06/04 تاریخ انتشار : 1403/07/04

کلید واژه: Ethephon, Daminozide, Phytochemical, Mucilage,

چکیده مقاله :

Chia (Salvia hispanica L.) is an annual plant of the Lamiaceae family that has commercial value due to its omega-3 and omega-6 content. Considering the limited cultivation of this plant in Iran, the effect of planting density and growth regulators under Gorgan weather conditions was investigated in this research. This research was conducted as a factorial design based on a randomized complete block design with 3 replications. The treatments included the cultivation distance (50, 60, and 70 cm), growth regulators including ethephon (0, 10, 30, and 60 mg), and daminozide (0, 25, 75, and 150 mg). The measured variables include morphological and yield traits (wet weight and dry weight of leaves, plant height, number of side branches, stem diameter, leaf area, number of inflorescences per plant) and phytochemical traits (total phenol, total flavonoid, mucilage, antioxidant activity by three methods (DPPH, TAOC, and FRAP)). Results indicate significant effects of ethephon and daminozide on plant growth, with differential impacts on morphological traits and reproductive growth. Specific combinations of ethephon and daminozide treatments influenced phytochemical composition, with notable variations in total phenol and flavonoid content. Antioxidant activity varied across treatments and assays, suggesting complex interactions between growth regulators and antioxidant metabolism. Additionally, mucilage production responded to growth regulator applications, with specific combinations yielding the highest mucilage content, notably with 30 ppm ethephon and 25 ppm daminozide at 70 cm row spacing. These findings contribute to optimizing chia cultivation practices and harnessing its potential as a functional food source with diverse health benefits.

چکیده انگلیسی:

Chia (Salvia hispanica L.) is an annual plant of the Lamiaceae family that has commercial value due to its omega-3 and omega-6 content. Considering the limited cultivation of this plant in Iran, the effect of planting density and growth regulators under Gorgan weather conditions was investigated in this research. This research was conducted as a factorial design based on a randomized complete block design with 3 replications. The treatments included the cultivation distance (50, 60, and 70 cm), growth regulators including ethephon (0, 10, 30, and 60 mg), and daminozide (0, 25, 75, and 150 mg). The measured variables include morphological and yield traits (wet weight and dry weight of leaves, plant height, number of side branches, stem diameter, leaf area, number of inflorescences per plant) and phytochemical traits (total phenol, total flavonoid, mucilage, antioxidant activity by three methods (DPPH, TAOC, and FRAP)). Results indicate significant effects of ethephon and daminozide on plant growth, with differential impacts on morphological traits and reproductive growth. Specific combinations of ethephon and daminozide treatments influenced phytochemical composition, with notable variations in total phenol and flavonoid content. Antioxidant activity varied across treatments and assays, suggesting complex interactions between growth regulators and antioxidant metabolism. Additionally, mucilage production responded to growth regulator applications, with specific combinations yielding the highest mucilage content, notably with 30 ppm ethephon and 25 ppm daminozide at 70 cm row spacing. These findings contribute to optimizing chia cultivation practices and harnessing its potential as a functional food source with diverse health benefits.

منابع و مأخذ:

Ayerza, R. and W. Coates. 2005. Chia: rediscovering a forgotten crop of the Aztecs. University of Arizona Press.
Biskup, I., I. Golonka, A. Gamian and Z. Sroka. 2013. Antioxidant activity of selected phenols estimated by ABTS and FRAP methods. Advances in Hygiene & Experimental Medicine/Postepy Higieny i Medycyny Doswiadczalnej, 67.
Chang, C.-C., M.-H. Yang, H.-M. Wen and J.-C. Chern. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis, 10, (3).
Di Sapio, O., M. Bueno, H. Busilacchi, M. Quiroga and C. Severin. 2012. Caracterización morfoanatómica de hoja, tallo, fruto y semilla de Salvia hispanica L.(Lamiaceae). Boletín latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 11, (3) 249-268.
Gardner, F. P., R. B. Pearce and R. L. Mitchell. 2017. Physiology of crop plants. Scientific publishers
Ghena, M. and M. B. Amany. 2020. Chia (Salvia hispanica L.) Seed oil a new source of omega-3. Plant Arch, 20, 2678-2683.
Grzeça, G. T., M. C. Winhelmann, A. A. Emer, M. Tedesco, M. Lazarotto and G. Schafer. 2021. Use of growth regulator in reducing the size of Sesbania punicea (Cav.) Benth. Acta Iguazu Cascavel Vol 10, n 2 (2021), p 40-47,
Hedden, P. and G. V. Hoad. 2021. Growth regulators and crop productivity. In Mechanisms of Plant Growth and Improved Productivity Modern Approaches:173-198: CRC Press. Number of 173-198 pp.
Jumrani, K. and V. S. Bhatia. 2018. Impact of combined stress of high temperature and water deficit on growth and seed yield of soybean. Physiology and Molecular biology of Plants, 24, (1) 37-50.
Knez Hrnčič, M., M. Ivanovski, D. Cör and Ž. Knez. 2019. Chia Seeds (Salvia hispanica L.): An overview—Phytochemical profile, isolation methods, and application. Molecules, 25, (1) 11.
Lee, S. E., H. J. Hwang, J.-S. Ha, H.-S. Jeong and J. H. Kim. 2003. Screening of medicinal plant extracts for antioxidant activity. Life sciences, 73, (2) 167-179.
Li, X., A. A. Thwe, C. H. Park, S. J. Kim, M. V. Arasu, N. Abdullah Al-Dhabi, S. Y. Lee and S. U. Park. 2017. Ethephon-induced phenylpropanoid accumulation and related gene expression in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) hairy root. Biotechnology & Biotechnological Equipment, 31, (2) 304-311.
Nadeem, F., M. A. Hanif, N. Almasoud, T. S. Alomar and A. Younis. E cient nanostructured materials to reduce nutrient leaching to overcome environmental contaminations.
Ritonga, F. N., D. Zhou, Y. Zhang, R. Song, C. Li, J. Li and J. Gao. 2023. The roles of gibberellins in regulating leaf development. Plants, 12, (6) 1243.
Sadeghi, S., A. Rahnavard and Z. Y. Ashrafi. 2009. The effect of plant density and sowing date on yield of Basil (Ocimum basilicum L.) in Iran. Journal of Agricultural Technology, 5, (2) 413-422.
Saptari, R. T., R. R. Esyanti and R. A. Putranto. 2022. Daminozide enhances the vigor and steviol glycoside yield of stevia (Stevia rebaudiana Bert.) propagated in temporary immersion bioreactors. Plant Cell, Tissue and Organ Culture (PCTOC), 149, (1) 257-268.
Sedghi, M., R. Seyed Sharifi, A. Namvar, T. Khandan-E-Bejandi and P. Molaei. 2008. Responses of sunflower yield and grain filing period to plant density and weed interference. Research Journal of Biological Sciences, 3, (9) 1048-1053.
Song, Y., C. Long, Y. Wang, Y. An and Y. Lu. 2024. Advancements in multi-omics for nutraceutical enhancement and traits improvement in buckwheat. Critical Reviews in Biotechnology, 1-26.
Sosa, A., G. Ruiz, J. Rana, G. Gordillo, H. West, M. Sharma, X. Liu and R. Torre. 2016. Chia crop (Salvia hispanica L.): its history and importance as a source of polyunsaturated fatty acids omega-3 around the world: a review. Journal of Crop Research and Fertilizers, 1, (104) 1-9.
Sun, L., J. Zhang, X. Lu, L. Zhang and Y. Zhang. 2011. Evaluation to the antioxidant activity of total flavonoids extract from persimmon (Diospyros kaki L.) leaves. Food and chemical toxicology, 49, (10) 2689-2696.
Ullah, A., H. Manghwar, M. Shaban, A. H. Khan, A. Akbar, U. Ali, E. Ali and S. Fahad. 2018. Phytohormones enhanced drought tolerance in plants: a coping strategy. Environmental Science and Pollution Research, 25, 33103-33118.
Vl, S. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178.
Yamazaki, M., A. Ishida, Y. Suzuki, Y. Aoki, S. Suzuki and S. Enoki. 2021. Ethylene induced by sound stimulation enhances anthocyanin accumulation in grape berry skin through direct upregulation of UDP-glucose: Flavonoid 3-O-glucosyltransferase. Cells, 10, (10) 2799.
Yan, H., H. Chen, M. Xia, Q. Liao, J. Zhao, L. Peng, L. Zou and G. Zhao. 2023. The Impacts of Plant Hormones on the Growth and Quality of Sprouts. Food and Bioprocess Technology, 1-30.
Yildirim, E., E. Özden and S. Ermis. 2024. Seed Biology-New Advances: New Advances. BoD–Books on Demand
Zade, S., P. Gourkhede, P. Vaidya, R. S. Singh, S. Sinha, A. Kumar and V. Kumar. 2021. Problem Soils And Their Management Practices.