مقایسه تجربی اثرات اسید اسکوربیک و تیامین در جلوگیری از ضایعات بافتی ناشی از سرب در برخی بافت‌های ماهی کپور (Cyprinus carpio)
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
فاطمه پورعلی
1
,
زهرا موسوی
2
,
داور شاهسونی
3
,
محمد عزیز زاده
4
1 - دانش آموخته دانشکده دامپزشکی، دانشگاه فردوسی مشهد، ایران
2 - گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران
3 - گروه بهداشت مواد غذایی و آبزیان دانشکده دامپزشکی دانشگاه فردوسی مشهد، مشهد، ایران.
4 - گروه علوم درمانگاهی - بهداشت و پیشگیری بیماریهای دامی دانشکده دامپزشکی دانشگاه فردوسی مشهد، مشهد، ایران.
تاریخ دریافت : 1396/05/21
تاریخ پذیرش : 1397/10/12
تاریخ انتشار : 1398/02/01
کلید واژه:
سرب,
اسید آسکوربیک,
ماهی کپور,
تیامین,
ضایعات بافتی,
چکیده مقاله :
با توجه به تجمع فلز سرب در بافت های خوراکی ماهی، امنیت و سلامت غذای انسان نیز تحت تاثیر قرار می گیرد. مطالعه حاضر به منظور مقایسه تجربی اثرات اسید آسکوربیک و تیامیندر جلوگیری از ضایعات بافتی ناشی از سرب در برخی بافت های ماهی کپور انجام شد. ماهیان به صورت تصادفی به 4 گروه 30تایی تقسیم شدند. گروه 1 به عنوان شاهد در نظر گرفته شد. گروه 2 استات سرب، گروه 3 و 4 علاوه بر سرب به ترتیب تیامین و اسید آسکوربیک نیز دریافت کردند. پس از کالبدگشایی، نمونه های بافتی از مغز، کبد و کلیه تهیه و برش های آماده شده، به روش هماتوکسیلین- ائوزین رنگ‌آمیزی شد. در مقایسه آماری ضایعات بافت مغز شامل پرخونی، ادم و تغییرات ایسکمیک در گروه 4 به صورت معنی داری کمتر از گروه های 2 و 3 بود. همچنین در کبد، پرخونی، هایپرپلازی کانون‌های ملانوماکروفاژ، تغییرات دژنراتیو و گنجیدگی های اسیدفست داخل هسته ای هپاتوسیت‌ها مشاهده گردید. شدت پرخونی در بافت کبد و تغییرات دژنراتیو هپاتوسیت ها در گروه 3 به صورت معنی داری کمتر از گروه 4بود. هیچ یک از شاخص های پاتولوژیک کلیه شامل پرخونی، خونریزی، گنجیدگی های اسیدفست داخل هسته ای، تورم و نکروز سلول های پوششی توبول ها و هایپرپلازی کانون های ملانوماکروفاژ، بین گروه های 3 و 4 تفاوت معنی داری نشان ندادند، اما در مقایسه برخی ضایعات این دو گروه با گروه 2، اختلاف معنی دار مشاهده گردید.بر پایه نتایج ذکر شده می توان نتیجه گیری نمود که اسید آسکوربیک و تیامین می توانند در پشیگیری و درمان مسمومیت با سرب به عنوان یک گزینه مناسب در ماهیان مطرح باشند.
چکیده انگلیسی:
Due to accumulation of lead in edible tissues of fish, the safety and health of human food are also affected. This study was conducted to compare the effects of ascorbic acid and thiamine in prevention of lead induced tissue damages in selected tissues of common carp (Cyprinus carpio). Fish were divided randomly into 4 groups of 30 fish each. Group 1 was considered as control group. Group 2 received lead acetate while groups 3 and 4 received thiamine and ascorbic acid respectively in addition to lead. After necropsy, tissue specimens were collected from the brain, kidney and liver and the processed slides were stained with hematoxylin and eosin. Brain lesions in group 4 consisting of hyperemia, edema and ischemic cell changes were significantly lower in comparison to groups 2 and 3. In liver, hyperemia, hyperplasia of melano-macrophage centers, hepatocellular degeneration and intranuclear acid-fast inclusion bodies were observed. The severity of hyperemia and degeneration in group 3 was significantly lower in comparison to group 4. In the statistical comparison, none of the renal pathological indices including hyperemia, hemorrhage, intranuclear acid-fast inclusion bodies, necrotic changes, swelling of epithelial cells and hyperplasia of melano-macrophage centersrevealed significant differences between groups 3 and 4 but compared to group 2, some lesions in these groups showed a significant difference. Based on the results, it can be concluded that ascorbic acid and thiamin might have some protective and therapeutic effects on lead poisoning in fish.
منابع و مأخذ:
Agius, C. and Roberts, R. (2003). Melano macrophage centres and their role in fish pathology. Journal of Fish Diseases, 26(9): 499-509.
Ajayi, G., Adeniyi, T. and Babayemi, D. (2009). Hepatoprotective and some haematological effects of Allium sativum and vitamin C in lead-exposed wistar rats. International Journal of Medicine and Medical Sciences, 1(3): 64-67.
Alvares, A.P., Fischbein, A., Sassa, Sh., Anderson, KE. and Kappas, A. (1976). Lead intoxication: Effects on cytochrome P450 mediated hepatic oxidations. Clinical Pharmacology and Therapeutics, 19(2): 183-190.
Al-Weher, S. (2008). Levels of heavy metal Cd, Cu and Zn in three fish species collected from the northern Jordan valley, Jordan. Jordan Journal of Biological Sciences, 1(1): 41-46.
Anbudhasan, P., Surendraraj, A., Karkuzhali, S. and Sathishkumaran, P. (2014). Natural antioxidants and its benefits. International Journal of Food and Nutritional Sciences, 3(6): 25-232.
Angelove, A., Hisitev, H. and Belchev L. (2002). Influence of ascorbic acid, thiamine or their combination on lead poisoning I albino rats. Bulgarian Journal of Veterinary Medicine, 5(4): 233-239.
Baker, J.C. (1987). Lead poisoning in cattle. Veterinary Clinics of North America: Food Animal Practice, 3(1): 137-147.
Booth, N.H. and McDonald, L.E. (1988). Veterinary pharmacology and therapeutics. Iowa State University Press, pp: 108-119.
Dalley, J., Gupta, P. and Hung, C. (1990). A physiological pharmacokinetic model describing the disposition of lead in the absence and presence of L-ascorbic acid in rats. Toxicology Letters, 50(2): 337-348.
Feller, W.R. (1998). Small Animal Toxicology and Poisonings. 1st ed., USA: Mosby, St Louis, pp: 167-172.
Fowler, B.A. and DuVal, G. (1991). Effects of lead on the kidney: roles of high-affinity lead-binding proteins. Environmental Health Perspectives, 91(2): 77-80.
Ghiasi, F. (2001). The Mechanism of Regulation of Metals in Fish. Tehran University, pp: 43-64. [In Persian]
Ghazi-Khansari, M., Hajighasemkhan, A. and Ghazaie, S. (1996). Influences of thiamine and/or ascorbic acid on lead intoxication. Acta Medica Iranica, 34(3-4): 61-64.
Halliwell, B., Wasil, M. and Grootveld, M. (1987). Biologically significant scavenging of the myeloperoxidase derived oxidant hypochlorous acid by ascorbic acid. Federation of the European Biochemical Societies (FEBS) Letters, 213(1): 15-17.
Jarrar, B.M. (2003). Histological and histochemical alterations in the kidney induced by lead. Annals of Saudi Medicine, 23(1-2): 10-15.
Järup, L. (2003). Hazards of heavy metal contamination. British Medical Bulletin, 68(1): 167-182.
Hayes, W. (2001). Principles and Methods of Toxicology. 4th ed., Philadelphia: Taylor and Francis, pp: 491-520.
Kalia, K. and Flora, S.J. (2005). Strategies for safe and effective therapeutic measures for chronic arsenic and lead poisoning. Journal of Occupational Health, 47(1): 1-27.
Khidr, B.M., Mekkawy, I.A., Harabawy, A.S. and Ohaida A.S. (2012). Effect of lead nitrate on the liver of the cichlid fish (Oreochromis niloticus): a light microscope study. Pakistan Journal of Biological Sciences, 15(18): 854.
Lukienko, P.I., Mel'nichenkoI, N.G., Zverinskii, S.V. and Zabrodskaya, V. (2000). Antioxidant properties of thiamine. Bulletin of Experimental Biology and Medicine, 130(3): 874-876.
Mokhtari, N.G. (2007). The effect of orally administered lead on thyroid gland hormones' and liver enzymes' concentrations in rats. Hormozgan Medical Journal, 11(2): 115-120. [In Persian]
Reddy, S.Y., Pullakhandam, R. and Kumar, B.D. (2010). Thiamine reduces tissue lead levels in rats: mechanism of interaction. Biometals, 23(2): 247-253.
Samad, L.H., Azizi, M. and Barzegar, M. (2007). Antioxidative effect of pomegranate seed phenolic componends on soybean oil. Journal of Agricultural Sciences, 14(4): 193-200.
Shahsavani, D., Movassaghi, A.R., Omid zahir, Sh. (2009). Therapeutic effect of thiamine on experimentally lead induced damages in Gold fish. Journal of Veterinary Research, 64(3): 237-242. [In Persian]
Shan, G., Tang, T. and Zhang, X. (2009). The protective effect of ascorbic acid and thiamine supplementation against damage caused by lead in the testes of mice. Journal of Huazhong University of Science and Technology, 29(1): 68-72.
Wang, C., Liang, J., Zhang, C., Bi, Y., Shi, X. and Shi, Q. (2007). Effect of ascorbic acid and thiamine supplementation at different concentrations on lead toxicity in liver. Annals of Occupational Hygiene, 51(6): 563-569.
Warren, C. (2001). Brush with death: a social history of lead poisoning. United States of America: John Hopkins University Press, pp: 27-40.
Ziegfeld, R.L. (1964). Importance and uses of lead. Archives of Environmental Health: An International Journal, 8(2): 202-212.
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Agius, C. and Roberts, R. (2003). Melano macrophage centres and their role in fish pathology. Journal of Fish Diseases, 26(9): 499-509.
Ajayi, G., Adeniyi, T. and Babayemi, D. (2009). Hepatoprotective and some haematological effects of Allium sativum and vitamin C in lead-exposed wistar rats. International Journal of Medicine and Medical Sciences, 1(3): 64-67.
Alvares, A.P., Fischbein, A., Sassa, Sh., Anderson, KE. and Kappas, A. (1976). Lead intoxication: Effects on cytochrome P450 mediated hepatic oxidations. Clinical Pharmacology and Therapeutics, 19(2): 183-190.
Al-Weher, S. (2008). Levels of heavy metal Cd, Cu and Zn in three fish species collected from the northern Jordan valley, Jordan. Jordan Journal of Biological Sciences, 1(1): 41-46.
Anbudhasan, P., Surendraraj, A., Karkuzhali, S. and Sathishkumaran, P. (2014). Natural antioxidants and its benefits. International Journal of Food and Nutritional Sciences, 3(6): 25-232.
Angelove, A., Hisitev, H. and Belchev L. (2002). Influence of ascorbic acid, thiamine or their combination on lead poisoning I albino rats. Bulgarian Journal of Veterinary Medicine, 5(4): 233-239.
Baker, J.C. (1987). Lead poisoning in cattle. Veterinary Clinics of North America: Food Animal Practice, 3(1): 137-147.
Booth, N.H. and McDonald, L.E. (1988). Veterinary pharmacology and therapeutics. Iowa State University Press, pp: 108-119.
Dalley, J., Gupta, P. and Hung, C. (1990). A physiological pharmacokinetic model describing the disposition of lead in the absence and presence of L-ascorbic acid in rats. Toxicology Letters, 50(2): 337-348.
Feller, W.R. (1998). Small Animal Toxicology and Poisonings. 1st ed., USA: Mosby, St Louis, pp: 167-172.
Fowler, B.A. and DuVal, G. (1991). Effects of lead on the kidney: roles of high-affinity lead-binding proteins. Environmental Health Perspectives, 91(2): 77-80.
Ghiasi, F. (2001). The Mechanism of Regulation of Metals in Fish. Tehran University, pp: 43-64. [In Persian]
Ghazi-Khansari, M., Hajighasemkhan, A. and Ghazaie, S. (1996). Influences of thiamine and/or ascorbic acid on lead intoxication. Acta Medica Iranica, 34(3-4): 61-64.
Halliwell, B., Wasil, M. and Grootveld, M. (1987). Biologically significant scavenging of the myeloperoxidase derived oxidant hypochlorous acid by ascorbic acid. Federation of the European Biochemical Societies (FEBS) Letters, 213(1): 15-17.
Jarrar, B.M. (2003). Histological and histochemical alterations in the kidney induced by lead. Annals of Saudi Medicine, 23(1-2): 10-15.
Järup, L. (2003). Hazards of heavy metal contamination. British Medical Bulletin, 68(1): 167-182.
Hayes, W. (2001). Principles and Methods of Toxicology. 4th ed., Philadelphia: Taylor and Francis, pp: 491-520.
Kalia, K. and Flora, S.J. (2005). Strategies for safe and effective therapeutic measures for chronic arsenic and lead poisoning. Journal of Occupational Health, 47(1): 1-27.
Khidr, B.M., Mekkawy, I.A., Harabawy, A.S. and Ohaida A.S. (2012). Effect of lead nitrate on the liver of the cichlid fish (Oreochromis niloticus): a light microscope study. Pakistan Journal of Biological Sciences, 15(18): 854.
Lukienko, P.I., Mel'nichenkoI, N.G., Zverinskii, S.V. and Zabrodskaya, V. (2000). Antioxidant properties of thiamine. Bulletin of Experimental Biology and Medicine, 130(3): 874-876.
Mokhtari, N.G. (2007). The effect of orally administered lead on thyroid gland hormones' and liver enzymes' concentrations in rats. Hormozgan Medical Journal, 11(2): 115-120. [In Persian]
Reddy, S.Y., Pullakhandam, R. and Kumar, B.D. (2010). Thiamine reduces tissue lead levels in rats: mechanism of interaction. Biometals, 23(2): 247-253.
Samad, L.H., Azizi, M. and Barzegar, M. (2007). Antioxidative effect of pomegranate seed phenolic componends on soybean oil. Journal of Agricultural Sciences, 14(4): 193-200.
Shahsavani, D., Movassaghi, A.R., Omid zahir, Sh. (2009). Therapeutic effect of thiamine on experimentally lead induced damages in Gold fish. Journal of Veterinary Research, 64(3): 237-242. [In Persian]
Shan, G., Tang, T. and Zhang, X. (2009). The protective effect of ascorbic acid and thiamine supplementation against damage caused by lead in the testes of mice. Journal of Huazhong University of Science and Technology, 29(1): 68-72.
Wang, C., Liang, J., Zhang, C., Bi, Y., Shi, X. and Shi, Q. (2007). Effect of ascorbic acid and thiamine supplementation at different concentrations on lead toxicity in liver. Annals of Occupational Hygiene, 51(6): 563-569.
Warren, C. (2001). Brush with death: a social history of lead poisoning. United States of America: John Hopkins University Press, pp: 27-40.
Ziegfeld, R.L. (1964). Importance and uses of lead. Archives of Environmental Health: An International Journal, 8(2): 202-212.
