Background & Aim: Knowledge of the traditional application of plants is declining faster than the plants themselves; this study was conducted to record and store the local knowledge and cultural outcomes for using the medicinal plants in treating diseases in Mobarakeh region, Isfahan, Iran. Experimental: This study has done based on applying the desert survey, information of local people, and identification, classification and introducing these plants. Then the plant species were collected and transferred to the department of botany in Isfahan Research Center for Natural Resources in order to be identified after drying and preparing their herbarium. Results & Discussion: Finally, 200 plant species were identified; from which 75 ones, belonged to 73 genera, and 33 families with medicinal properties, were identified. From this number, 75% are herbaceous, ten percent shrubby and 15 percent are arboreous. The highest number of medicinal species belongs to the Lamiaceae family with nine species, Asteraceae family with eight species, Rosaceae family with six species, and the families Apiaceae, Papilionaceae, and Brassicaceae with five species. These plants are used in traditional medicine for treatment of gastrointestinal ailments, relaxation, cough, diarrhea, low blood sugar, and hyperlipidemia. Recommended applications/industries: According to results of this study, the preservation and conservation of some herbs species is essential. This document can play a role in preserving the indigenous knowledge of using these plants. پرونده مقاله

Background & Aim:Oxidative stresses caused by free radicals are known to promote many diseases such as inflammation and cancer; therefore, any plant product that can inhibit free radical production can play an important role in preventing diseases. We aimed to compare the fracture rate and the scavenging activity of free radicals, and the antioxidant composition of rolB-transformed hairy root of Catharanthus roseus with those of the plants natural root and leaf. Experimental: Hairy root of the Madagascar periwinkle (C. roseus) were induced using Agrobacterium rhizogenes strain ATCC 15834 to transfer the rolB gene. PCRanalysis was used to identify the geneʼs presence in the transformed hairy root. Folin-Ciocalteu reagent and HPLC were used to determine total phenolics and gallic acid. This study also entailed rapid screening of antioxidant by dot-blot DPPH staining on thin-layer chromatography (TLC), radicals fracture rate, free radical scavenging by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, and nitric oxide-scavenging activities by Griess reagent. Results: Our results showed that phenolic and gallic acid content in the ethanol extract of hairy root was significantly higher (p ≤ 0.05) than that naturally found in the root and leaf of C. roseus. The hairy root extract had the highest dot-blot staining and fracture rate of radicals and the lowest IC50 for inhibition of DPPH• and •NO. The transgenic hairy root of C. roseus showed high number of scavenging radicals, whereas the most active ethanol extract of hairy root registered by the highest level of gallic acid and total phenolic content. Recommended applications/industries: The transformed hairy roots can be considered as a rich natural source of antioxidants. پرونده مقاله