The development of biocompatible packaging is a fine replacement for using of synthesis film. The purpose of the presentation investigation was to prepare and characterize biocompatible film. The active films were fabricated from polylactic acid (PLA) containing roselle calyx extract (RCE) and roselle calyx extract (RCE)/halloysite nanoclay (HNC). The incorporation of HNC and extract improved the functional characteristics of films. RCE and HNC/RCE incorporated films represented lower oxygen permeability (OP) than control film, 1.82×10-18, 1.41×10-18, and, 3.61×10-18 m2 s-1 Pa -1 respectively. Water vapor permeability (WVP) and thickness of PLA film, PLA/RCE, and PLA/RCE/HNC film were estimated as follows: 3.61×10-14, 2.5×10-14, and 1.46×10-14kg m m-2s-1Pa-1; 0.18, 0.21, and 0.22 mm respectively. The tensile strength and young modulus of the films increased from 47.91 to 62.83 MPa and 9.4 to 17.4 MPa respectively by the addition of extract and RCE/HNC into the biodegradable film. This investigation illustrated films based on PLA incorporated RCE/HNC and RCE has positive functional properties for applications in natural film packaging. پرونده مقاله