شناسایی ژنوتیپ‌های مقاوم به تنش خشکی در هندوانه و ارزیابی پاسخ آن‌ها با توجه به محدود بودن منابع آبی در کشور یک اولویت تحقیقاتی محسوب می‌شود. به منظور ارزیابی پاسخ فیزیولوژیک و زراعی توده‌های هندوانه محلی علی‌آباد، سیستانی و تربت به تنش خشکی، آزمایشی به صورت کرت‌های خرد شده در قالب بلوک‌های کامل تصادفی دارای سه رژیم آبیاری 100، 70 و 40 درصد نیاز آبی که به ترتیب به عنوان تیمارهای عدم تنش، تنش خفیف و تنش شدید تقسیم بندی شدند، در بهار و تابستان 1397 در منطقه جیرفت اجرا شد. بیشترین مقدار عملکرد در سطوح 100 و 70 درصد نیاز آبی بود و کمترین آن در سطح 40 درصد نیاز آبی مشاهده شد. در تیمار عدم تنش و رژیم آبیاری 70 درصد بین توده‌ها از لحاظ عملکرد، محتوای رطوبت نسبی و مقدار پرولین تفاوت معنی‌داری وجود نداشت. با افزایش تنش و اعمال رژیم آبیاری 40 درصد نیاز آبی، توده سیستانی عملکرد فیزیولوژیک و زراعی بهتری از خود نشان داد. با افزایش مقدار تنش محتوای پرولین و فعالیت آنزیم‌های آنتی‌اکسیدانی سوپر اکسید دسموتاز و کاتالاز در تمامی توده‌ها افزایش یافت که این مقدار افزایش در هندوانه سیستانی بیشتر از توده‌های دیگر بود. با توجه به نتایج این مطالعه می‌توان اظهار داشت که توده سیستانی با حفظ عملکرد فیزیولوژیکی و زراعی در شرایط تنش نسبت به دیگر هندوانه‌ها مقاومت بهتری نشان داد. استفاده از توده‌های محلی و متحمل هندوانه در برنامه‌های اصلاحی و مدیریت بهینۀ آبیاری می‌تواند به کشاورزان در رویارویی با کمبود آب کمک کند. پرونده مقاله

Background & Aim: Lemon verbena (Lippia citriodora H.B.K.) is a richly scented herb and has a long history as a sacred and medicinal plant. Select an appropriate medium in tissue culture is a necessity for success Experimental: To evaluate the effect of 10 medium of previous researches for Lippia micropropagation (1- MS medium supplemented with BAP (3 mgL-1) + IBA (0.1 mgL-1) + activated charcoal (1 gL-1) 2- MS medium supplemented with BAP (1 mgL-1) + IBA (0.5 mgL-1) + activated charcoal (1 gL-1) 3- 1/2MS 4- MS medium supplemented with BAP (5 mgL-1) 5- MS medium supplemented with BAP (1 mgL-1) 6- MS medium supplemented with activated charcoal (1 gL-1) 7- MS medium supplemented with BAP (1 mgL-1) + activated charcoal (1 gL-1) 8- MS medium supplemented with BAP (1 mgL-1) + AgNO3 (5 mgL-1) 9- MS medium supplemented with AgNO3 (5 mgL-1) 10- MS (control)), an experiment was conducted as a Completely Randomized Design (CRD) with three replicates and three explants per replication. Results & Discussion: The results showed positive effects for BAP (5 mgL-1) and somewhat active charcoal, but contrary to predictions, AgNO3 had no effect on reducing senescence and leaf necrosis. Recommended applications/industries: Therefore, Based on the finding, the four medium (MS medium supplemented with BAP (5 mgL-1)) with the highest rates of shoot fresh weight, leaf number, internodes number, shoot number, root fresh weight, callus diameter and root dry weight can be introduced as a suitable medium of tissue culture in this experimental conditions. پرونده مقاله

In this study, the possibility of organogenesis of leaves and petioles of two African violet cultivars were evaluated using MS media containing various concentration of plant growth regulators (IAA (1,2 mg/l), BA (0.05, 0.1, 0.2, 0.5 mg/l), NAA (1, 2 mg/l), and TDZ (0.01 mg/l)). All experiments were arranged completely randomized design then data analyzed by one-way ANOVA, and means were separated using Duncan’s new multiple range test (𝑃 ≤ 0.01). Direct and indirect organogenesis from the whole leaf with petiole bases were observed in both cultivars. However, the separated leaf blade and petiole were unable to produce any organs. The organogenesis potential of cultivars was significantly different, although the medium containing BA (0.1 mg/l) + IAA (1 mg/l) showed the highest indirect organogenesis (90 %< in both cultivars). In contrast, the media containing BA 0.05 mg/l + TDZ 0.01 mg/l + IAA 2 mg/l and BA 0.05 mg/l + TDZ 0.01 mg/l + IAA 1 mg /l had more success on the induction of direct organogenesis. Therefore, the level of direct and indirect organogenesis appears to be dependent on the interaction between the PGRs and the explant type having a direct correlation with the genotype. پرونده مقاله

The study presents flavonoids compounds patterns of thirty two Lamiaceae (Mint) taxa from Oshtorankoh located on Zagros Mountains, Iran for understanding flavonoids role in mint chemotaxonomy and their usages as food additive, edible, spices and medicine. This is a novel report of some Iranian Mint taxa flavonoids using two-dimensional paper chromatography and thin layer chromatography methods. Results showed all of the studied taxa contained flavone C-&C-/O-glucosides and flavonoid sulphates. Eight taxa had aglycones while the rest lacked. Quercetin was found in all of taxa except Lamium album ssp. crinitum and Nepeta persica. Stachys setifera had not myricetin while others had. Rhamnetin, tricin and morin were not detected in all taxa exceptional Salvia brachycalyx and Salvia staminea those had. Kaempferol was found in all taxa except Ajuga chamaecistus, Lamium amplexicaule var. amplexicaule, Nepeta persica and Stachys pilifera. All of taxa except six species had luteolin. These results showed aerial parts flavonoids compounds variation in studied taxa can be useful for studying relationships within relatively narrow taxonomic limits, e. g. at the species and genus levels and their importance in chemotaxonomic surveys of mint genera. Also flavonoids compounds presence in studied taxa increase their quality and antioxidant activity as edible, spices and medicinal plants. پرونده مقاله

In almonds (Prunus dulcis), selecting salt-tolerant rootstocks and genotypes is an appropriate breeding strategy. In the present research, we grafted two commercial almond cultivars (‘Sahand’ and ‘TS3’) on the ‘GF677’ rootstock. Then, we monitored the impact of salinity (0.5, 6.5, and 8.5 dS m-1) on the morphological, physiological, and molecular characteristics of the Sahand and TS3 cultivars. The photosynthetic rate, chlorophyll (a, b and total) content, and carotenoid content decreased with increasing salinity levels in both cultivars, with the least decrease observed in TS3. Under a salinity level of 8.5 dS/m, Sahand exhibited the lowest growth (8.9 cm), leaf area (5412.5 mm2), Chla, Chlb, Chltotal and carotenoid contents (0.58, 0.15, 0.74 and 0.31 mg g-1FW, respectively). Additionally, Sahand had a Fm/Fv (0.75), N content (1.33%) and Ca, B, Mg, S, Fe and Zn values of 1654.55, 1.64, 395.28, 168.6, 10.35 and 3.05 mg L-1, respectively. Furthermore, Sahand exhibited the highest MDA level (25.17 nmol g-1FW), TFC (2.95 mg GA g−1FW), Na content (649.84 mg L-1) and Cl content (3.52%). the lowest TFC (1.75 mg GA g−1FW) and the highest NHX1 expression, photosynthesis rate (5.65 μmol m-2 s-1), gs (0.1 mol m-1 s-1) transpiration rate (6.08 mmol m-1 s-1), Ca, S and B content (1903.63, 196.9 and 2.09 mg L-1, respectively) were belonged to TS3 under 0.5 dS/m salinity. Higher levels of Mg and Fe in the TS3 cultivar resulted in the stablization of photosynthetic pigments. Compared to Sahand, TS3 had a higher nitrogen content, and its greater NHX1 expression was a molecular confirmation of its salt tolerance. پرونده مقاله