Quorum sensing (QS) is a bacterial communication mechanism that regulates the production of many pathogenic factors, including the formation of pigments and the ability to form biofilms that are essential for chronic infections. For discovering new inhibitors on the formation of biofilm formation, over 700 synthetic and natural compounds have been virtually screened against the triphenyl-LasR enzyme involved in Pseudomonas aeruginosa’s QS system. The 3D-QSAR studies revealed the relationship between the quantitative structure of compounds and their activity. The drug-like properties of compounds and effective pharmacophore features on the ligand interaction with protein were investigated by ADME and E-pharmacophore analysis. According to the obtained results, we identified compound with PubChem ID 118732838 with the glide score of -12.34 kcal/mol and oral absorption of 58.75% as the potential compound for inhibiting triphenyl-LasR protein. The study outcomes can help us to identify new drugs to inhibit biofilm formation and decrease bacterial resistance. پرونده مقاله

Vitis vinifera L. with common name of grape is native to Mediterranean region and today is grown all over the world.آHere, the adsorption/desorption process on LXA-10 resin was investigated to enrich polyphenolic compounds of grape extract. The extract was obtained using ultra-sound-assisted probe with water and ethanol (50:50) with an extraction yield of 26.4%. The pH value of the extract for maximum adsorption was optimized at 3.26. According to the findings of this study, the kinetic adsorption fitted well with pseudo-second-order model (R2=0.99). Moreover, the dynamic study revealed the adsorption capacity and required solvent for polyphenolic desorption being 42 mL and six-bed volume (BV), respectively. In the enriched powder, the amount of quercetin-O-3-glucuronide and quercetin-O-3-glucoside increased from the ranges of 19.43 to 45.92 mg.g-1 and 41.244 to 72.88 mg.g-1, respectively. The antioxidant activity of enriched polyphenols also increased from 252.37 in initial grape extract to 408.03 mM iron (II).mg-1. پرونده مقاله