Liver diseases are most commonly occurring nowadays, that’s why we are in argent need to develop new strategies in treatment. to evaluate the role of MSCs in regenerating liver cells and to clarify the anti-inflammatory role of HO-1 either alone or as a combined therapy with MSCs. 72 rats were divided into seven groups (n=10 rats/group) as follows, group1: control rats, group 2: CCL4, group 3: CCL4 that received MSCs group 4: CCL4 that received HO-1 inhibitor, group 5: CCL4 that received HO-1 inducer, group 6: CCL4 that received combined MSCs and HO-1 inhibitor , and group 7: CCL4 that received combined MSCs and HO-1 inducer. All groups were evaluated histopathologically with assessment of liver functions. The combined MSCs and HO-1 inducer group showed the highest significant results in ALT (p-value ˂0.05), albumin (p-value ˂0.05), HO-1 activity (p-value ˂0.0001), and genes expression compared to other groups. This is due to the cumulative anti-inflammatory role of both MSCs and HO-1 together with the ability of MSCs to increase the HO-1 expression with further reduction in inflammation and fibrosis. MSCs and HO-1 inducer provide promising tool in treatment of liver disease. پرونده مقاله

Colorectal carcinoma (CRC) is a leading cause of death especially in industries worker. The aim of this study is to identify the role of heavy elements exposure on CRC DNA methylation.The study was conducted on 25 CRC patients. Biopsies were taken by colonoscopy from malignant tissue and adjacent normal tissues for comparative assessment of BRAF/KRAS, methylated MLH1 and MGMT between the normal and malignant tissues by using real time PCR. In an attempt to identify wither heavy metal like Lead, Aluminum and Mn have a role in cancer development or not, we compared their levels in the serum of 25 CRC patients and 25 normal volunteers by using atomic absorption. The expressions of BRAF/KRAS, methylated MLH1 and MGMT were significantly higher in malignant tissues compared to normal tissues (p value<0.001). Additionally, the levels of lead and aluminum but not Mn were statistically significantly increased in CRC patients compared to normal controls (p value<0.001). Lead and Aluminum were positively correlated with all studied parameters. Heavy metals act as starting signals for carcinogenesis through DNA methylation. پرونده مقاله

Diabetes mellitus and obesity are a growing overall medical issues, particularly in developed nations. Vitamin D deficiency is pandemic that has been involved in a different kind of disease. This study was intended to detect the effect of vitamin D deficiency and vitamin D receptor polymorphisms on type 2 diabetes mellitus (T2DM) patients through induction of obesity. 80 Egyptians T2DM patients and 20 non diabetic subjects were included in our research. Diabetic cases were categorized into two subgroups according to diabetic complications. PCR-RFLP for vit D receptor polymorphisms and vit D level were assessed to all subjects. The deficiency of vitamin D was more prevalent in diabetic cases than in control cases. There was significant difference between control and diabetic patients in genotyping of FokI (VDR 2228570 C) and TaqI (VDR rs731236 T) polymorphisms. The results of our study revealed that vitamin D deficiency could be a contributing factor for T2DM rising among Egyptian patients. Additionally, there was a genetic variation in the VDR genes FokI and TaqI related to diabetic mellitus in Egyptian patients. پرونده مقاله