City growth and environmental difficulties last decades leads the art of urban design and development think moreprecisely and new approaches for conserving environment and developing urban areas have been evolved.The present study dealswith concepts related to ecology in the city and in particular the theory of Ecological Urbanism and its criteria is expressed. Alsotheory and characteristics of cultural landscape is described. Persian traditional architecture and urban design especially in historicurban fabric has unique criteria, Shushtar ancient urban fabric is investigated in this research and its extraordinary characteristics arecompared with theories mentioned above.This paper is based on quantitative and qualitative research methods reviewed recent andreliable articles and documents. Also case study is assessed by reviewing, historical books and different official documents in additiona complete assessment of case study is performed by site survey, socioeconomic survey, physical survey, and demographic analysis.Plenty of data gathered by questionnaires, interviewing officials, authorities and residents. Result came out from comparison theoriesand case study indicates that Shushtar has both ecological urbanism and cultural landscape values and it could be argue that culturallandscape are special features of the culture, art and relation between human and nature in each society, they were existed long beforethe eco urbanism criteria formed, yet, it seemed there are some similar criteria to define them that illustrate the inseparable fact andrelation between them. پرونده مقاله