Study of composition and antimicrobial effects of Thymus carmanicus, Zataria multiflora, Rosmarinus Officinalis and Cinnamomum verum essential oils on Aeromonas hydrophila

Subject Areas : Food Microbial Contamination

Manouchehr Momeni shahraki ¹ , Reza Sharafati Chaleshtori ² , Mehdi Raissy ³ , Mahsa Ansari ⁴ , Fatemeh Heidarinezhad Chaharmahali ⁵ , Farhang Tirgir ⁶

1 - Department of Food Hygiene, School of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran
3 - Department of Aquatic Animal Health, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
4 - Department of Fisheries, Agricultural Jahad, Shahrekord, Iran. Young Researchers Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
5 - Bachelor of Genetics, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
6 - Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Accepted : 0621-03-23 Published : 2021-12-12

Keywords: Essential oils, Antibacterial, Aeromonas hydrophila,

Abstract :

Aeromonas hydrophila is an important pathogenic agent in aquatics and zoonotic in humans. This pathogenic agent can extensively be found in water and also is a part of fishes' digestive system microflora. Extensive researches have been carried out on the antimicrobial effects of natural compounds against food-borne pathogens, indicating that these products can substitute antibiotics. Essential oils of Thymus carmanicus, Zataria multiflora, Cinnamomum verum, Rosmarinus officinalis have antimicrobial effects due to their phenolic compounds. In this study, the bioactive compounds were detected using Gas Chromatography equipped with the mass spectrometer. Then minimum inhibitory concentrations and minimum bactericidal concentrations of the essential oils against A. hydrophila were calculated. According to the results, the lowest and the highest MIC and the minimum MBC were observed in T. carmanicus and Z. multiflora, respectively. The result indicated that the inhibition zone diameter increased with the increase of essential oil concentration. The inhibition zone, in the concentration of 1500 ug mL-1 T. carmanicus was 24.33±0.57 mm. The findings of this study showed the antimicrobial resistance of these compounds. Considering the increasing limitations against chemicals and antibiotics, such compounds could be recommended for prevention.

References:

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