Molecular assay of chicken meat fraud in premium burgers by Simplex and Duplex PCR

Subject Areas : Food Science and Technology

M. Hashemzadegan ¹ (M.Sc. Graduate in Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran)
E. Hosseini ² (Associated Professor of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran)
F. Tafvizi ³ (Associated Professor of Biology, Parand Branch, Islamic Azad University, Parand, Iran)
M. Bayat ⁴ (Associated Professor of Microbiology, Sciences and Researches Branch, Islamic Azad University, Tehran, Iran)

Received: 2017-02-28 Accepted : 2018-03-11 Published : 2018-06-22

Keywords: Chicken Meat, Simplex-PCR, Premium hamburger, Duplex-PCR,

Abstract :

Meat is considered an excellent and nutritious source of protein. However, due to the high cost of this animal protein and economic profit access of some producers, frauds like usage meat species of commercially lower value are noticeable. The aim of this study is to identify low-cost protein frauds such as chicken meat by the molecular method. DNA was extracted from 10 kinds of industrial premium beef burgers, and control samples involved raw beef and chicken meat. Then, simplex-PCR and duplex-PCR were run on the extracted DNA using specific primers of cytochrome b gene in beef and 12s rRNA gene in chicken. Specific genes of beef cytochrome b and chicken 12s rRNA amplified 274 bp and 183 bp fragments respectively in all premium burger samples in simplex-PCR and duplex-PCR. Amplification of fragments of 183 bp by simplex and duplex-PCR suggests adulteration of premium hamburgers by partial replacement of beef with chicken.

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